Transcriptome-guided Gene Isolation, Characterization and Expression Analysis of a Phosphomevalonate Kinase Gene (GBPMK) from Ginkgo Biloba. | [International Journal of Agriculture and Biology • 2018]

Author(s):

1. Qiling Song: College of Horticulture and Gardening, Yangtze University, Jingzhou, 434025, China

2. Xiangxiang Meng: College of Horticulture and Gardening, Yangtze University, Jingzhou, 434025, China

3. Yongling Liao: College of Horticulture and Gardening, Yangtze University, Jingzhou, 434025, China

4. Weiwei Zhang: College of Horticulture and Gardening, Yangtze University, Jingzhou, 434025, China

5. Jiabao Ye: College of Horticulture and Gardening, Yangtze University, Jingzhou, 434025, China

6. Feng Xu: College of Horticulture and Gardening, Yangtze University, Jingzhou, 434025, China

Abstract:

Terpenoids are main active ingredients of Ginkgo biloba. Phosphomethoxylate kinase (PMK) is one of the core enzyme in the mevalonate pathway, one of the two pathways in plants that can synthase terpenoids. In this study, a novel PMK gene (designated as GbPMK) was cloned from G. biloba. The expression profile of GbPMK in different tissues (roots, stems, leaves, male strobili, female strobili, and fruits) and under the treatments of hormones and stresses were investigated by qRTPCR. Bioinformatics analysis indicated that the cDNA sequence of GbPMK contained a 1557-bp open reading frame encoding 519 amino acids. Protein structure analysis showed that the GbPMK protein had four conserved domains and one conserved region of the GHMP kinase family. Phylogenetic tree analysis revealed that GbPMK possessed a conserved sequence structure and sequence characteristics. The qRT-PCR analysis results showed that GbPMK exhibited tissue-specific expression, with the highest expression in leaves and the lowest expression in male strobili. GbPMK exerted different degrees of response to treatments with MeJA, ABA, Eth, SA, dark and low temperature but did not respond to wound treatment. The characterization and expression analysis of GbPMK contributed to understanding the biosynthesis of terpenoids at the molecular level. This study provides molecular information for the future through genetic engineering techniques to improve the yield of terpenoids in G. biloba.

Page(s): 1080-1088

DOI: 10.17957/IJAB/15.0612

Published: Journal: International Journal of Agriculture and Biology, Volume: 20, Issue: 5, Year: 2018

Keywords:

Keywords are not available for this article.

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