The Immune Response and Passive Protective Abilities of the Outer Membrane Lipoprotein Slp of Aeromonas hydrophila against the Major Pathogenic Bacteria of Freshwater Aquaculture in Fish | [Pakistan Journal of Zoology • 2024]

Author(s):

1. Sijie Jian: Chinese-German Joint Institute for Natural Product Research, College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China; Shaanxi Province Key Laboratory of Bio-Resources, Hanzhong 723000, China; QinLing-Bashan Mountains Bioresources Comprehensive Development C.I.C., Hanzhong 723000, China; Qinba State Key Laboratory of Biological Resources and Ecological Environment, Hanzhong 723000, China

2. Wei Sun: Chinese-German Joint Institute for Natural Product Research, College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China; QinLing-Bashan Mountains Bioresources Comprehensive Development C.I.C., Hanzhong 723000, China

3. Jia Chao: Chinese-German Joint Institute for Natural Product Research, College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China; Shaanxi Province Key Laboratory of Bio-Resources, Hanzhong 723000, China

4. Na Rong: Chinese-German Joint Institute for Natural Product Research, College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China; Qinba State Key Laboratory of Biological Resources and Ecological Environment,Hanzhong 723000,China

5. Xiang Liu: Chinese-German Joint Institute for Natural Product Research, College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China; Shaanxi Province Key Laboratory of Bio-Resources, Hanzhong 723000, China; QinLing-Bashan Mountains Bioresources Comprehensive Development C.I.C., Hanzhong 723000, China; Qinba State Key Laboratory of Biological Resources and Ecological Environment, Hanzhong 723000, China

6. Chen Chen: Chinese-German Joint Institute for Natural Product Research, College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China; Shaanxi Province Key Laboratory of Bio-Resources, Hanzhong 723000, China; QinLing-Bashan Mountains Bioresources Comprehensive Development C.I.C., Hanzhong 723000, China; Qinba State Key Laboratory of Biological Resources and Ecological Environment, Hanzhong 723000, China

Abstract:

Aeromonas hydrophila and Pseudomonas fluorescens are major pathogenic bacteria in freshwater aquaculture and cause huge economic losses. The outer membrane lipoprotein Slp of A. hydrophila has potential applications in fish vaccines. Slp bioinformatics analysis showed that anti-Slp serum might provide cross-protection to resist bacterial infection in fish. Slp was obtained by molecular cloning, expression and purification, and the expression conditions were optimized. In mice immunized with Slp, the immunerelated factors of LZM and AKP were enhanced (p < 0.05), and a specific antiserum titer (1: 3200) was obtained that had immune recognition effects for A. hydrophila and P. fluorescens. Passive immunization of Carassius auratus with Slp mouse serum and challenging with bacteria showed a passive protection rate of Slp serum against A. hydrophila infection of 42.5 % (p < 0.05) and a passive cross-protection rate against P. fluorescens of 18.6 %; the immune-related factors of LZM, AKP and ACP and the leukocyte phagocytosis of phagocytic percentage (PP) and phagocytic index (PI) increased (p < 0.05); the inflammation-related genes expression of IL-6, IL-8, IL-1ß and TNF-a decreased (p < 0.05) in kidneys and spleens after challenging with A. hydrophila and P. fluorescens; and reduced injury to the kidneys, spleens and intestines; there was no significant change in the antioxidant-related factors of MDA, SOD, CAT and GSH-PX. Therefore, Slp has passive protection (A. hydrophila) and passive cross-protection (P. fluorescens) abilities and it can boost resistance to infection using major freshwater aquaculture etiologic agent of fish.

Page(s): 877-889

DOI: 10.17582/journal.pjz/20211005111000

Published: Journal: Pakistan Journal of Zoology, Volume: 56, Issue: 2, Year: 2024

Keywords:

fish , Fish , vaccine , Aeromonas hydrophila , Passive immune protection , Pseudomonas fluorescens

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