A NEW SPECIES OF Clitopilus Shanglayensis (ENTOLOMATACESAE, AGARICALES) REPORTED FROM KHYBER PAKHTUNKHWA, PAKISTAN | [Pakistan Journal of Weed Science Research • 2022]

Author(s):

1. Saif Ur Rehman: Department of Botany, Hazara University,Mansehra,Pakistan

2. Muhammad Faiz: Department of Botany, Hazara University,Mansehra,Pakistan

3. Abdul Nisar Khalid: Department of Botany, University of the Punjab,Lahore,Pakistan

4. Muhammad Binyamin Khan: Department of Botany, Hazara University,Mansehra,Pakistan

5. Ali Hazrat: Department of Botany, University of Malakand,Chakdara,Pakistan

6. Muhammad Yahya: Department of Botany, University of Malakand,Chakdara,Pakistan

7. Muhammad Ajmal Khan: Center of Biotechnology and Microbiology, University of Peshawar,KP.,Pakistan

Abstract:

In current study a new species Clitopilus shanglayensis (Entolomatacesae, Agaricales) was reported based on morphological and molecular confirmation from northern region of Pakistan. Morphological parameters which differ the species from other species include white yellowish pileus (05-06 cm wide), light brown stipe and pale yellowish lamella. The fungal molecular markers nuclear ribosomal internal transcribed spacer (ITS) and larger subunits (LSU) were sequenced. These sequences were further used for evolutionary analysis through various bioinformatics tools. The phylogenetic analysis based on ITS and LSU sequences by using Maximum Likelihood method (ML) revealed that the novel species reported from current study had distinct characteristics features than other species. A complete detail description, color photographs and evolutionary relatedness of novel species Clitopilus shanglayensis were mentioned.

Page(s): 159-167

DOI: 10.28941/pjwsr.v28i2.1001

Published: Journal: Pakistan Journal of Weed Science Research, Volume: 28, Issue: 2, Year: 2022

Keywords:

molecular , Agaricales , Phylogenetic analysis , Entolomatacesae , Clitopilus

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