Abstract:
Prebiotics, non-digestible compounds that enhance beneficial gut microbiota, have gained attention for their role in improving gut microbiome health. Mucilage from Salvia hispanica (chia), rich in polysaccharides, is a promising dietary component with multiple health benefits. The current research aimed to produce bioactive oligosaccharides from chia seed mucilage and evaluate in vitro prebiotic effects on human probiotics. Polysaccharides extracted from chia seed mucilage were precipitated with 75% ethanol, digested by Pichia pastoris, and purified via size exclusion chromatography. Biochemical analysis revealed that fraction ChF78 had the highest unsaturated uronic acid (7.79 ± 0.71 mg/mL), fraction EPP had the highest uronic acid (5.57 ± 0.32 mg/mL) and pentose (6.54 ± 0.30 mg/mL), while fraction ChF90 had the highest hexose content (4.60 ± 0.12 mg/mL). Prebiotic effects were notable in fractions ChF91 and ChF90, which significantly enhanced the proliferation of Bifidobacterium bifidum and Bifidobacterium clausi, achieving cell counts of 9.11 ± 0.17 log10 CFU/mL and 9.96 ± 0.30 log10 CFU/mL, respectively, at 48 hours. The acidifying effect was strongest in EPP and ChF91 fractions, with pH levels dropping to 4.14 ± 0.13 and 4.46 ± 0.28. Prebiotic index and score analyses further highlighted ChF91 and ChF90 for their potent prebiotic potential. The prebiotic analysis highlighted ChF91 with the highest scores for both B. bifidum (5.67 ± 0.13) and B. clausi (5.47 ± 0.23), while ChF90 showed a strong prebiotic index (2.15 ± 0.07) for B. bifidum and (3.25 ± 0.12) for B. clausi. These findings support the potential for developing functional foods, paving the way for novel dietary interventions and functional food development to enhance gut health.
Page(s):
117-117
DOI:
DOI not available
Published:
Journal: 1st International Conference on "Recent Advances in Green Biotechnology and Climate Resilience", September 15-16, 2025, Volume: 1, Issue: 1, Year: 2025
Keywords:
probiotics
,
Pichia pastoris fermentation
,
Oligosaccharides
,
Chia seed mucilage