Exploring genetic potential of rice accessions for salinity tolerance at seedling stage using phenotypic and molecular markers | [1st International Conference on "Recent Advances in Green Biotechnology and Climate Resilience", September 15-16, 2025 • 2025]

Author(s):

1. Naeem Akhtar: Department of Plant Breeding and Genetics, College of Agriculture, University of Sargodha, Pakistan

2. Raees Ahmad Mughal: Department of Plant Breeding and Genetics, College of Agriculture, University of Sargodha, Pakistan

3. Usman Saleem: Department of Plant Breeding and Genetics, College of Agriculture, University of Sargodha, Pakistan

4. Ikram ul Haq: Department of Plant Breeding and Genetics, College of Agriculture, University of Sargodha, Pakistan

5. Talat Mahmood: Department of Plant Breeding and Genetics, PMAS Arid Agriculture University, Rawalpindi, Pakistan.

6. Muhammad Mazhar Iqbal: Department of Soil and Environmental Sciences, College of Agriculture, University of Sargodha. Pakistan.

Abstract:

Rice (Oryza sativa L.) is a staple food of about half the population on earth. It is salt sensitive crop and most of the rice growing area in Pakistan is saline to saline sodic soil. Therefore, there is a need to screen our existing rice germ plasm for its salinity tolerance. Glasshouse studies were conducted to evaluate advanced rice breeding lines against salinity. The 15 rice lines including two commercial rice varieties (Super Basmati and KSK282) were grown in hydroponic system and were evaluated for both morphological as well as molecular levels. Four salinity levels i.e. 0 dSm-1, 4 dSm-1, 8 dSm-1 and 12 dsm-1NaCl. This displayed enough genetic variation in rice genotypes for salinity tolerance under reported salinity levels. Furthermore, ten DNA based SSRs markers named as RM1287, RM10800, RM3412, RM10, RM10793927,RM140, RM7075 and RM7414 were used to locate Saltol QTL (located on chromosome 1) which creates salinity tolerance/resistance in rice cultivars. Results showed that Genotype OPS-132 performed really well in both physical assessment (performing moderate tolerant to SES examination of genotypes against salinity) as well as molecular level (8 out of 10 markers showed positive band in OPS-132). OPS-132 also performed better than check varieties in all aspects and suggested that this genotype should be include in rice hybridization program to develop salt tolerance cultivars.

Page(s): 145-145

DOI: DOI not available

Published: Journal: 1st International Conference on "Recent Advances in Green Biotechnology and Climate Resilience", September 15-16, 2025, Volume: 1, Issue: 1, Year: 2025

Keywords:

Oryza sativa , SSRs , Hydroponic , Saltol QTL , KSK 282

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