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Single nucleotide polymorphism and phylogenetic analysis of bambara groundnut (vigna subterranea (L.) verdc) cpDNA rpoA Gene
Author(s)
Abubakar Bilyamini Mu'azu Department of Biochemistry, Faculty of Basic Medical Sciences, Bayero University,P. M. B. 3011, Kano,Nigeria; Department of Biochemistry, Faculty of Science, Yobe State University Damaturu,P. M. B. 3011, Kano,Nigeria
Yusuf Y. Muhammad Department of Biochemistry, Faculty of Basic Medical Sciences, Bayero University, P. M. B. 3011, Kano, Nigeria
Abdullahi Abdul Kadir Imam Department of Biochemistry, Faculty of Basic Medical Sciences, Bayero University, P. M. B. 3011, Kano, Nigeria
Abstract
Bambara groundnut (Vigna subterranea (L.) Verdc), a legume native to Africa, has the potential to address food security challenges on the continent. However, it is an underutilized crop because there hasn't been enough research done on its genetic diversity, selection, and breeding, particularly with the use of genomic tools. This study was aim at assessing the genetic diversity in chloroplast genome of Bambara groundnut genotypes by using agarose gel electrophoresis and single nucleotide polymorphism (SNPs). The results showed the PCR gel products of the amplified rpoA gene is ± 1000 base pairs (bp) for the seven Vigna subterranea genotypes. A total of 442 and 437 SNPs were detected in 5'UTR and 3'-UTR regions from the amplified sequence of seven genotypes of Vigna subterranea rpoA gene which represent 4.2 and 4.4%.
Publication Details
Page(s) 202-202
DOI DOI not available
Published Journal: Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 , Volume: 0, Issue: 0, Year: 2023
Keywords
phylogenetic analysis bambara groundnut SNPs cpDNA rpoA
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