Teratogenic and embryo-toxic effects of latex and leaves methanol extract of euphorbia helioscopia l. in mice and chicken embryos. | [Journal of Animal and Plant sciences • 2014]

Author(s):

1. U. Saleem: University College of Pharmacy, University of the Punjab, Lahore, Pakistan

2. B. Ahmad: University College of Pharmacy, University of the Punjab, Lahore, Pakistan

3. M. Ahmad: University College of Pharmacy, University of the Punjab, Lahore, Pakistan

4. K. Hussain: University College of Pharmacy, University of the Punjab, Lahore, Pakistan

5. N. I. Bukhari: University College of Pharmacy, University of the Punjab, Lahore, Pakistan

6. B. Ahmad: Faculty of Pharmacy, The University of Lahore, Lahore, Pakistan

7. A. A. Anjum: Department of Microbiology, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan

8. U. Saleem: College of Pharmacy, Government College University, Faisalabad, Pakistan

Abstract:

Euphorbia helioscopia L. has been investigated for antibacterial, antifungal, antiviral and vasodepressor activities. The study was designed to probe into the reproductive toxicity (teratogenicity) potential of Euphorbia helioscopia L. in albino mice. Toxicity of latex and methanol extract of leaves (10, 50, 80, 100 and 120 µg/200 µL) was evaluated in white leghorn chicken embryos (5th day) using chorioallantoic membrane route (n = 30 for each experiment i.e. latex & extract, or 5 per dose and 5 as control group). Teratogenic potential of latex (n= 5) and extract (100, 300, 500, 700, 1000, 1500 and 2000 mg/kg body weight, n= 40, or 5 per concentration and 5 as control group) was evaluated in pregnant albino mice on 7th day of gestation using intra-peritoneal route. Both latex and leave extract checked in chicken embryos were safe. LD50 calculated with TSK software was 1397 mg/kg body weight for methanol extract of leaves observed in albino mice. The rest of the experimental concentrations were non-lethal in mice without any observable malformation. It is concluded that the concentration (administered parentally) less than LD50 is devoid of teratogenic effects in pregnant female mice and chicken embryos.

Page(s): 450-454

DOI: DOI not available

Published: Journal: Journal of Animal and Plant sciences, Volume: 24, Issue: 2, Year: 2014

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