Abstract:
Cellulases play a pivotal role as hydrolytic enzymes responsible for breaking down cellulose into glucose. They are crucial for biofuel production and for waste, textile, and food processing industries. However, industrial applications are limited by low stability, poor enzymatic reusability, and high production costs. Using nanomaterial’s for enzyme immobilization offers enhancement in catalytic and operational stability. In this work, cellulase from Aspergillus nigerwas immobilized on silver-gold hybrid (Ag Au) nanoparticles which were synthesized using Northern catalpa leaf extract. Structural, compositional, and morphological characterization of the Nano hybrids was done using FTIR, XRD,SEM. Kinetic studies on free and immobilized cellulase comparisons showed the difference in Vmax and Km parameters, which showed increased catalytic activity and substrate affinity post-immobilization. In addition, the immobilized enzyme showed increased thermal and pH stability as well as increased reusability in multi-cycle operation. Permanent hydrolysis of cellulose were observed as continuous glucose was produced, which indicates a significant amount of cellulase remains functional in the system. This study offers a novel and environmentally friendly approach for cellulase immobilization with increased efficiency which may be utilized in biofuel production and other biotechnological applications.
Page(s):
87-87
DOI:
DOI not available
Published:
Journal: 1st International Conference on "Recent Advances in Green Biotechnology and Climate Resilience", September 15-16, 2025, Volume: 1, Issue: 1, Year: 2025