Abstract:
Probiotics, beneficial microorganisms known for their positive impact on human health, require specific nutrients to thrive and exert their health-promoting effects. Oligosaccharides, a class of dietary carbohydrates, have gained attention as potential prebiotics, selectively nourishing beneficial gut microbiota. The current study aimed to isolate ethanol-precipitated oligosaccharides from cress seed mucilage and evaluate their in vitro prebiotic effects on human probiotics. Mucilage was extracted by immersing cress seeds in autoclaved distilled water overnight, and polysaccharides were precipitated in 75% ethanol. The ethanolprecipitated polysaccharides were digested to produce bioactive oligosaccharides using Pichia pastoris fermentation. The fermented product was fractionated using size-exclusion chromatography. Ethanol-precipitated polysaccharides (EPP) substantially supported the growth of P. pastoris, with the cell count rising from 4.98 ± 0.13 log CFU/mL at 0 hours to 8.76 ± 0.17 log CFU/mL after 6 hours, followed by a decline over 72 hours. The biochemical contents of fractions were analyzed. Results revealed that DF73 contained the highest carbohydrate content (3.14246 mg/mL). EPP contained the highest unsaturated uronic acid (5.54436 ± 0.35138 µg/g) and hexose (6.54849 ± 0.30852 µg/g) contents. DF58 exhibited the highest uronic acid concentration (7.98201 ± 0.73763 µg/g), while DF72 had the highest pentose content (4.54738 ± 0.11939 µg/g). The prebiotic effect of the selected carbohydrate-rich fractions was determined on probiotics, including Bacillus clausi and Bifidobacterium bifidum. DF73 displayed the strongest acidifying effect with a pH drop to 4.74333 ± 0.27538 after 24 hours. DF48 showed a potent acidifying effect on B. clausii with a pH of 4.34 ± 0.04 at 24 hours. In terms of bacterial proliferation, DF73 supported significant growth of B. bifidum from (2.88649 ± 0.13948) to (9.96 ± 0.26) log10 CFU/mL at 48 hours) and B. clausii from (2.8595 ± 0.13948) to (9.75598 ± 0.4967) log10 CFU/mL at 48 hours). DF72 and DF73 demonstrated the highest prebiotic scores and indices, with DF73 exhibiting a prebiotic score of (5.66789 ± 0.12713) for B. bifidum and (5.47309 ± 0.237) for B. clausii. These findings revealed the potential of cress seed-derived polysaccharides, particularly fractions DF72 and DF73, as effective prebiotics for the growth of beneficial gut microbiota and highlighted the importance of improving functional foods to promote gut health.
Page(s):
45-45
DOI:
DOI not available
Published:
Journal: 1st International Conference on "Recent Advances in Green Biotechnology and Climate Resilience", September 15-16, 2025, Volume: 1, Issue: 1, Year: 2025