Targeting Diabetic Wound Healing Through Tissue Regeneration with Silver, Copper, and Zinc Nanoparticles in Streptozotocin-Induced Diabetic Albino Rat Model | [Pakistan Veterinary Journal • 2025]

Author(s):

1. Ghulam Murtaza: Faculty of Veterinary and Animal Sciences, Gomal University, Dera Ismail Khan, KPK, Pakistan; Department of Anatomy, University of Agriculture, Faisalabad, Pakistan

2. Razia Kausar: Department of Anatomy, University of Agriculture,Faisalabad,Pakistan.

3. Shahnawaz Sial: Department of Comparative Biomedical Sciences. University of Surrey. UK

4. Amjad Hameed: Nuclear Institute of Agriculture and Biology,Faisalabad,Pakistan

5. Sarmad Rehan: Department of Anatomy, University of Agriculture,Faisalabad,Pakistan.

6. Aneela Kanwal: Faisalabad Medical University and Allied Teaching Hospital,Faisalabad,Pakistan

7. M. Umar Sharif: Department of Anatomy, University of Agriculture,Faisalabad,Pakistan.

Abstract:

Diabetic wound healing remains a significant clinical challenge, necessitating the development of more effective therapeutic strategies. In this study, we explored the potential of topically applied, chemically synthesized nanoparticles (NPs) as a novel therapeutic strategy for diabetic wound healing. Specially, we evaluated the efficacy of these NPs based ointments on diabetic wound healing through tissue regeneration, using streptozotocin-induced diabetic albino rat model. For this purpose, thirty-six male albino rats were divided into six groups, T0 (Control Normal, CN), T0 (Diabetic Control, DC), T1 (treated with AgNPs @3mg/ml), T2 (treated with CuNPs @2mg/ml), T3 (treated with ZnNPs @5mg/ml), and T4 (treated with Ag/Cu/Zn nanocomposite @3.5mg/ml). The diabetes was induced by intraperitoneal injection of streptozotocin (@40mg/kg body weight) in experimental rats. Subsequently, a full thickness 10mm diabetic wound was induced on the dorsal lumber region of each rat, and treated with AgNPs, CuNPs, ZnNPs, and Ag/Cu/Zn nanocomposite-based ointments. The results revealed significant improvements (P<0.05) in wound contraction rate and reduced healing time in the T4 group treated with Ag/Cu/Zn nanocomposite ointment. Furthermore, histological analysis also showed a significant (P<0.01) effect on the formation of new epithelium, granulation tissue, angiogenesis, and collagen deposition in the T4 group treated with Ag/Cu/Zn nanocomposite ointment, resulting in diabetic wound regeneration. In conclusion, the Ag/Cu/Zn nanocomposite ointment not only promotes diabetic wound healing but also significantly improves the structural integrity of the regenerated tissue, highlighting its potential as an effective therapeutic option for diabetic wounds.

Page(s): 1244-1252

DOI: 10.29261/pakvetj/2025.223

Published: Journal: Pakistan Veterinary Journal, Volume: 45, Issue: 3, Year: 2025

Keywords:

AgNPs CuNPs Diabetic Wound NanoMedicine Tissue Regeneration ZnNPs

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