Article Detail — Pakistan Science Abstracts

Choose Theme

Theme 1 — Green & Gold

No Detail Found!
Accumulation of Viral Coat Protein in Chloroplasts of Lily Leaves Infected with Lily Mottled Virus.
Author(s)
Pinsan Xu School of Life Science and Biotechnology, Dalian University of Technology, Dalian Liaoning 116024, China: School of Life Science and Medicine, Dalian University of Technology, Panjin Liaoning 124221, China
Xiuying Xia School of Life Science and Biotechnology, Dalian University of Technology, Dalian Liaoning 116024, China
Jiong Song School of Life Science and Biotechnology, Dalian University of Technology, Dalian Liaoning 116024, China
Zhengyao Zhang School of Life Science and Medicine, Dalian University of Technology, Panjin Liaoning 124221, China
Abstract
The symptoms of Lily mottled virus (LMoV) disease are thought to be caused by metabolic changes in leaf chloroplasts. To observe variations in the ultrastructure of cells and the accumulation of viral coat protein (CP) in lily leaves, we examined ultrathin sections of lily leaves infected by LMoV. Immunogold labeling analysis demonstrated that LMoV-CP was localized to the chloroplasts. The chlorophyll fluorescence parameters of LMoV-infected lily, which indicating that the accumulation of LMoV-CP in chloroplasts inhibits PSII activity. We investigated the transmembrane transport of LMoV CP by incubating a gradient of this protein. The lowest concentration of LMoV-CP detected was 30 µg mL-1 and the optimal incubation time was 1 min. High levels of LMoV-CP that accumulate inside chloroplasts may affect photosynthesis in virus-infected lily by inhibiting photosystem activity.
Publication Details
Page(s) 1265-1269
DOI 10.17957/IJAB/15.0436
Published Journal: International Journal of Agriculture and Biology, Volume: 19, Issue: 5, Year: 2017
Keywords
Keywords are not available for this article.
References
Benetti , M.P.,Tomassoli, 1988.Research on lily symptomless virus in Italy: purification and serology,Informatore Fitopatologico 38 51 -55
Chun , Y.U.,Y.J.Fan,Y.J.Zhou, 2008.Characteristics of Importing Tobacco Mosaic Virus Coat Protein into Intact Chloroplasts in vitro and Its Species Specificity,Jiangsu J. Agric. Sci. 24 810 -814
Cline , K.,M.Werner-Washburne,J.Andrews,K.Keegstra, 1984.Thermolysin is a suitable protease for probing the surface of intact pea chloroplasts,Plant 75 675 -678
Dekker , E.L.,A.F.Derks,C.J. ASJES,M.E.Lemmers,J.F.Bol,S.A.Langeveld, 1993.Characterization of Potyviruses from Tulip and Lily which Cause Flower-Breaking, 74 881 -887
Fu , D.Y.,J.Hong,J.S.Chen,J.X.Wu, 2004.Accumulation of coat protein of Turnip mosaic virus in host chloroplasts and its effect on PS II activity,Acta Photophysiol 30 34 -40
Ivanov , K.I.,K.Mäkinen, 2012.Coat proteins, host factors and plant viral replication,Curr. Opin. Virol. 2 712 -718
Korbin , M.,M.Kaminska, 1998.Characterization of cucumber mosaic cucumovirus isolates,Plant 48 95 -100
Li , Y.,M.Y. Wu , H.H.,Song , X.,Hu and B.S.,Qiu, 2005.Identification of a tobacco protein interacting with tomato mosaic virus coat protein and facilitating long-distance movement of virus,Arch Virol 150 1993 -2008
Reinero , A.,R.N.Beachy, 1989.Reduced Photosystem II Activity and Accumulation of Viral Coat Protein in Chloroplasts of Leaves Infected with Tobacco Mosaic Virus, 89 111 -116
Ren , C.M.,Z.B.Cheng,L.H.Wei,Y.J.Fan,Y.J.Zhou, 2012.Analysis on the Import of Coat Proteins of Chinese Wheat Mosaic Virus and Barley Yellow Mosaic Virus into Intact Chloroplasts in vitro,J. Triticeae Crops 32 163 -167
Shintaku , M., 1991.Coat protein gene sequences of two cucumber mosaic virus strains reveal a single amino acid change correlating with chlorosis induction,J. General Virol 72 2587 -2589
Xu , P.,J. Jia , B. L.,Sun and X,Jiang, 2014.Effects of Lily symptomless virus on photosynthesis, activities of defense-related enzymes and chloroplast ultra-structure in lily,Acta Phytopathol 44 387 -392
Xu , Y.,D.Hu,Y.Fang, 1998.Preparation of immuno-gold probes for plant virus identification,J. Zhejiang Agric. Univ., S1 29 -32
Yamaji , Y.,Lu , X.,Kagiwada , S.,Oshima and O.,Namba, .Molecular evidence that a lily-infecting strain of Tulip breaking virus from Japan is a strain of Lily mottle virus,Eur. J. Plant Pathol 107 833 -837
Zhu , S.,F.Francki, 1992.Cucumber Mosaic Virus Coat Protein Inside the Chloroplast of the Infected Tobacco, 3 328 -333
Citations
Citations are not available for this document.
0

Citations

0

Downloads

75

Views

Copyright ©  PASTIC National Center, Islamabad www.pastic.gov.pk