Author(s):
1. Muhammad Yasir Ali:
Faculty of Pharmaceutical Sciences, GC University Faisalabad, Faisalabad, Pakistan
2. Udo Bakowsky:
Dept. Pharmaceutics & Biopharmaceutics, University of Marburg, Marburg, Germany
Abstract:
Targeting the cancer cells has always been one of the major goals of chemotherapy to reduce the toxicity of cytotoxic substances. Among various drug delivery systems DPPC–based Liposomes are the most widely used and commercially successful carrier systems for this purpose. Their ability to encapsulate a great variety of drugs, high bioavailability and biocompatibility have made them suitable for surface modification with ligands with binding affinity against different cellular sites. In this study, DPPC liposomes prepared by film hydration evaporation technique in presence of Sorafenib (SFB) as chemotherapeutic agent. The surface of these liposomes, with or without drug, was decorated with aptamer (Apt) against HER3 using EDC/NHS chemical modification. Liposomes were characterized with regard to their size and shape by dynamic light scattering and atomic force microscopy. Particles were analyzed for cell base assessment in breast cancer line, MDA-MB-231. Cell viability assay, pathway analysis and apoptosis assay showed cellular toxicity in the presence of aptamer (p < 0.001). Metastatic inhibition showed reduced cell proliferation in the presence of Apt and SFB. Confocal laser scanning microscopy was used for the visualization of the receptor-mediated time-dependent intracellular uptake along with distribution of the modified nanoparticles throughout the cytoplasm. The findings of the current study confirmed the usefulness of the surface decorated SFB-loaded particles against HER3.
Page(s):
303-303
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
Modification
,
In vivo
,
Sorafenib
,
in vitro
,
Aptamer
References:
References are not available for this document.
Citations
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