Author(s):
1. Madieha Ambreen:
Botany Department, Minhaj University, Lahore, Pakistan
2. Safdar Ali Mirza:
Botany Department, Minhaj University, Lahore, Pakistan
Abstract:
A number of diseases are related to oxidative stress caused by free radicals produced during metabolism. Free radicals cause changes in the function and structure of nucleic acids and proteins, lead to cause diseases. In present work, free radical scavenging and anticancer activity of tannins in A. aspera callus cultures has been investigated. Callus induction efficiency was good for leaf and root explants showing callus index up to 160 as compared to stem explant under the influence of auxins. Extraction yield of callus tannins was higher in chloroform extracts whereas total antioxidant activity was comparable in chloroform, methanol and petroleum ether callus extracts followed by petroleum ether extract of said medium. Chloroform extract of stem callus culture grown on medium MSDN showed the highest total antioxidant activity. Tannins from A. aspera leaf callus extract had significant anticancer effect against the Jurket cell line as determined in the way of cell viability; anticancer activity was higher than other callus cultures but comparable to stem tissue. We may conclude that the in vitro biomass production could help to enhance the synthesis of bio active compounds like tannins from selected medicinal plants. Tannins extracted from A. aspera can be important in development of new medicines.
Page(s):
35-35
DOI:
DOI not available
Published:
Journal: Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 , Volume: 0, Issue: 0, Year: 2023
Keywords:
oxidative stress
,
Callus culture
,
scavenging activity
,
cell viability
,
tannins content
References:
References are not available for this document.
Citations
Citations are not available for this document.