Author(s):
1. Naureen Akhtar:
Institute of Agricultural Sciences, University of the Punjab,Lahore,Pakistan
2. Rahila Hafeez:
Institute of Agricultural Sciences, University of the Punjab,Lahore,Pakistan
3. Amna Ali:
Institute of Agricultural Sciences, University of the Punjab,Lahore,Pakistan
Abstract:
Mucorales (Zygomycetes) are among the most ancient groups of fungi. Although they are fast growing in culture but preservation of these cultures is rather sensitive as compared to other filamentous fungi. First Fungal Culture Bank of Pakistan maintained a large number of isolates of this group. Recently viability of members of mucorales was assessed that were isolated and purified from a variety of substrates between the years 2003-2006. Agar slants of pure cultures of these strains were preserved at 4 °C. These include seven species each of the genus Absidia and Mucor, four species of Rhizopus, two of Cunninghamella and one species belonging to genus Phycomyces. Results indicated that most of the strains lost their viability during storage period even none of the species from genus Absidia and Phycomyces survived. Therefore maintaining cultures of mucorales by this method is not recommended for their long term preservation. For long term preservation of fungal cultures, cryopreservation, lyophilization or silica gel preservation could be the alternate methods.
Page(s):
147-152
DOI:
DOI not available
Published:
Journal: Pakistan Journal of Phytopathology, Volume: 28, Issue: 2, Year: 2016
Keywords:
stability
,
preservation
,
viability
,
Fungal culture
References:
References are not available for this document.
Citations
Citations are not available for this document.