Topoisomerase Mutations Are Associated with High-level Ciprofloxacin Resistance in Staphylococcus saprophyticus, Enterococcus faecalis and Escherichia coli Isolated from Ducks. | [Pakistan Veterinary Journal • 2018]

Author(s):

1. Guo Ji: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 1 Xujiaping, Yanchangbao, Lanzhou 730046, China

2. Qiwei Chen: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 1 Xujiaping, Yanchangbao, Lanzhou 730046, China

3. Xiaowei Gong: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 1 Xujiaping, Yanchangbao, Lanzhou 730046, China

4. Fuying Zheng: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 1 Xujiaping, Yanchangbao, Lanzhou 730046, China

5. Shengdou Li: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 1 Xujiaping, Yanchangbao, Lanzhou 730046, China

6. Yongsheng Liu: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 1 Xujiaping, Yanchangbao, Lanzhou 730046, China

Abstract:

This study investigated the effects of ciprofloxacin on specie and resistance of the bacteria isolated from the ducks reared in ciprofloxacin-present and ciprofloxacinfree duck population. Bacteria were isolated from the carcass swab samples collected from the slaughtered ducks. The result showed that the dominant bacteria isolated from the ducks were Macrococcus caseolyticus (M. caseolyticus), Staphylocuccus saprophyticus (S. saprophyticus), Enterococcus faecalis (E. faecalis) and Escherichia coli (E. coli). The minimum inhibitory concentrations (MICs) of ciprofloxacin against all isolates were measured, and the genes of topoisomerase II (DNA gyrase) and topoisomerase IV of S. saprophyticus, E. faecalis and E. coli were amplified and sequenced, respectively. The results showed that the site-directed mutations of DNA gyrase and topoisomerase IV of the above three bacteria caused the strains to generate high-level resistance to ciprofloxacin (MIC=32 µg/ml). No plasmid-mediated quinolone resistance (PMQR) genes were detected. These results suggested that ciprofloxacin could affect bacterial species in the duck and induce bacterial resistance. Site-directed mutations of DNA gyrase and topoisomerase IV of S. saprophyticus, E. faecalis and E. coli were closely related to high-level ciprofloxacin resistance.

Page(s): 39-45

DOI: DOI not available

Published: Journal: Pakistan Veterinary Journal, Volume: 38, Issue: 1, Year: 2018

Keywords:

DNA gyrase Mutation Topoisomerase IV

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