A cytological study on the meiosis process of humulus scandens | [Pakistan Journal of Botany • 2024]

Author(s):

1. JINJIN GUO: Engineering Research Center of Crop Genetic Improvement and Germplasm Innovation in Henan Province, College of Life Sciences, Henan Normal University,Xinxiang ,China

2. DEQING MENG: Engineering Research Center of Crop Genetic Improvement and Germplasm Innovation in Henan Province, College of Life Sciences, Henan Normal University,Xinxiang 453007,China

3. DEQING MENG: Engineering Research Center of Crop Genetic Improvement and Germplasm Innovation in Henan Province, College of Life Sciences, Henan Normal University,Xinxiang 453007,China

4. MENGYI WANG: Engineering Research Center of Crop Genetic Improvement and Germplasm Innovation in Henan Province, College of Life Sciences, Henan Normal University,Xinxiang 453007,China

5. YANING LIU: Engineering Research Center of Crop Genetic Improvement and Germplasm Innovation in Henan Province, College of Life Sciences, Henan Normal University,Xinxiang 453007,China

6. GEYANG WU: Engineering Research Center of Crop Genetic Improvement and Germplasm Innovation in Henan Province, College of Life Sciences, Henan Normal University,Xinxiang 453007,China

7. JINHONG YUAN: Engineering Research Center of Crop Genetic Improvement and Germplasm Innovation in Henan Province, College of Life Sciences, Henan Normal University,Xinxiang 453007,China

8. JUNHUA LI: Engineering Research Center of Crop Genetic Improvement and Germplasm Innovation in Henan Province, College of Life Sciences, Henan Normal University,Xinxiang 453007,China

Abstract:

Humulus scandens is a common dioecious weed that possesses valuable forage and medicinal properties. It exhibits strong adaptability and a high seed yield, making it a subject of significant research interest. However, there is a lack of information concerning the meiosis process of H. scandens and the relevant methods used for studying it. In this study, we aimed to address this gap by utilizing intact H. scandens meiosis cells as research material. We employed a rapid labeling method to observe the meiotic chromosomes. Additionally, a single oligonucleotide probe of 5S rDNA was used to asertain that the number of 5S rDNA signals in the somatic cells of H. scandens was one pair. Using this probe, we conducted an inspection of homologous chromosome pairing in H. scandens. Our study provides a comprehensive description of the meiosis process in H. scandens and introduces a set of cytological methods suitable for the easy labeling of meiotic chromosomes and the monitoring of homologous chromosome pairing in H. scandens.

Page(s): 2245-2250

DOI: 10.30848/PJB2024-6(40)

Published: Journal: Pakistan Journal of Botany, Volume: 56, Issue: 6, Year: 2024

Keywords:

fish , Chromosome , Fish , Meiosis , Cytogenetics , Humulus scandens , 5S rDNA

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