Cotton leaf curl disease (CLCuD) epidemic in early 90s posed a serious threat to the fibre-based economy of Pakistan. All commercially grown varieties showed different levels of susceptibility to the disease. Attempts to screen breeding material were hampered due to lack of reliable screening methods. A simple lab-based technique has been developed to identify resistance against Cotton Leaf Curl Virus (CLCuV) in elite commercial cotton varieties and breeder’s material in the pipeline. CLCuV is transmitted by whitefly in a persistent manner therefore, the strategy employed whitefly-mediated transmission in insect-proof cages. Susceptible genogypes produced typical CLCuV) symptoms upon inoculation. Virus presence in all genogypes was tested by triple antibody sandwich enzyme linked immunosorbent assay (TAS-ELISA). Virus concentration in all susceptible genogypes was determined one month post-inoculation at ten days interval. Further indexing of symptomless and ELISA negative genotypes was confirmed by graft inoculation and Polymerase Chain Research (PCR). After successful graft establishment, five genotypes (CIM 4346, CIM 443, CIM 446 & CIM 448, CIM 1100) produced hardly visible sporadic speck of vein thickening and were found negative by ELISA and PCR. This technique has tremendous potential for exploring inherent resistance in advance lines throughout the year.