Acute toxicity and antinociceptive activity of saponins rich fraction of dioscorea deltoidea (WALL) | [Khyber Medical University Journal • 2020]

Author(s):

1. Niaz Ali: Department of Pharmacology, Institute of Basic Medical Sciences (IBMS), Khyber Medical University,Peshawar,Pakistan

2. Nur-Ul-Ain: Department of Pharmacology, Institute of Basic Medical Sciences (IBMS), Khyber Medical University,Peshawar,Pakistan

3. Muhammad Nabi: Department of Pharmacology, Institute of Basic Medical Sciences (IBMS), Khyber Medical University,Peshawar,Pakistan

4. Zakia Subhan: Department of Pharmacology, Institute of Basic Medical Sciences (IBMS), Khyber Medical University, Peshawar, Pakistan. Department of Pharmacology, Khyber Medical University Institute of Medical Sciences,Kohat,Pakistan

5. Shakir Ullah: Department of Pharmacology, Institute of Basic Medical Sciences (IBMS), Khyber Medical University,Peshawar,Pakistan; Department of Pharmacy, University of Malakand, Chakdara, Dir Lower, Pakistan

6. Ulfat Sultana: Department of Pharmacology, Institute of Basic Medical Sciences (IBMS), Khyber Medical University, Peshawar, Pakistan. Department of Pharmacology, Rehman Medical College, Peshawar, Pakistan

7. Bilal Shams: Department of Pharmacy, University of Peshawar, Peshawar, Pakistan

Abstract:

OBJECTIVE: To evaluate the saponins rich fraction of Dioscorea deltoidea (D. deltoidea) for possible antinociceptive activity. METHODS: Saponins were extracted from the crude methanolic extract of D. deltoidea (Wall). Presence of saponins was confirmed through phytochemical screenings. Acute toxicity test was performed to determine safe dose range of the saponins rich fraction (n-butanol fraction) using balb C Mice. The saponins rich fraction was assessed for possible antinociceptive activity using acetic acid induced writhing method and hot plate method. Data was analyzed using Graph Pad Prism 6. ANOVA was used to determine the significance of test samples versus positive control at 95% CI with p<0.05. RESULTS: The results showed that crude saponin rich fraction was safe up to test dose of 1000 mg/kg administered orally. In acetic acid induced writhing method, its test samples in doses of 10 and 50 mg/kg showed respectively 75.51% and 85.71% inhibition of writhing, while diclofenac sodium showed 74.4% inhibition of writhing. Percent inhibition of latency time of test samples increased from 87.87% to 133.6% in test doses of 10 mg/kg to 100 mg/kg dose, respectively, while Tramadol showed latency time of 85.12% within 30 minutes of its administration. CONCLUSION: Saponins rich n-butanol fraction of Dioscorea deltoidea (D.deltoidea) showed significant antinociceptive activity.

Page(s): 107-112

DOI: 10.35845/kmuj.2020.19787

Published: Journal: Khyber Medical University Journal, Volume: 12, Issue: 2, Year: 2020

Keywords:

nHexane , chloroform , Ethylacetate , Analgesics , Writhing , Dioscorea deltoidea , Acetic Acid , Tramadol , Diclofenac Sodium , Nbutanol

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