Abstract:
The marvels of DNA recombination technology have revolutionized the field of biotechnology. Several hormones, antibody subunits, vaccines, enzymes, and interferons are being produced at the industrial level, in suitable expression systems, under optimized conditions. For recombinant protein production, a range of expression systems are available such as bacteria, yeast, fungi, plant cells, insects and animal cells, etc. All recombinant proteins are naturally diferent from each other and various challenges are kept into consideration while choosing an expression system for their production. Every expression system has its advantages and limitations on the basis of which it can be considered or rejected for a particular protein production. Therefore, it is very significant to investigate the potential and limitations of several expression systems to choose the suitable one for particular protein production at an industrial scale. The optimization criteria of an expression system is evaluated on several factors such as productivity, eficiency, physiological characteristics, total cost, safety, convenience, and down-streaming conditions. Escherichia coli and Saccharomyces cerevisiae remained the organisms of choice to produce recombinant proteins for a long time, but now several other microorganisms are also being targeted to evaluate their eficiency toward recombinant protein production. Prokaryotic expression systems can be used to produce eukaryotic proteins as well however, the use of a eukaryotic expression system is preferable because it retains the structural, functional, and regulatory properties of therapeutic proteins. This review illustrates a brief view of a variety of expression systems, their eficiency, and limitations in recombinant protein production.
Page(s):
5-27
DOI:
DOI not available
Published:
Journal: Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences, Volume: 60, Issue: 1, Year: 2023
Keywords:
cell lines
,
DNA recombinant technology
,
Recombinant Proteins
,
Expression Systems