Abstract:
The effect of low closes of fast neutrons on the DNA genome of broad bean (Vicia faba) mature seeds was determined. In this study, inter simple sequence repeat (ISSR)-polymerase chain reaction (PCR) tool was used to analyze the genetic alteration of treated seeds of V. faba using six ISSR primers, Results showed that each primer gave rich and clear patterns with bands ranged from 179 to 1256 hp, Among the 61 ISSR fragments, 21 were representing the effect of the treatment of V. faba seed samples. ISSR markers clearly and easily distinguish allthe tested samples and also discriminate between fast neutron treated and untreated seeds. The dissimilarity values ranged 42 to 47% between the fast neutron-treated seeds and the control seed (untreated) and from 27 to 38% between the fast neutron-treated seeds. The dendrogram resulting from a UPGMA cluster analysis comprised two main distinct clusters, the first one including the control V. faba seed (untreated) and the second, includes all samples from the same kind of treatment divided into three groups: the S4 (group A), S2 and S3 (group B) and S I (group C), these groups being separated at a similarity level.
Page(s):
31-39
DOI:
DOI not available
Published:
Journal: Pakistan Journal of Biotechnology, Volume: 4, Issue: 1--2, Year: 2007