Vaccination with irradiated tachyzoites show better therapeutic effect than some plants or drugs on toxoplasmosis. | [Pakistan Journal of Zoology • 2016]

Author(s):

1. Mervat Abdel Fattah Moawad: Health Radiation Research Department, National Centre for Radiation Research and Technology, P. O. Box 29 Nasr City, Egypt

2. Mona Mohamed Amin: Health Radiation Research Department, National Centre for Radiation Research and Technology, P. O. Box 29 Nasr City, Egypt

3. Eman Naser Hafez: Health Radiation Research Department, National Centre for Radiation Research and Technology, P. O. Box 29 Nasr City, Egypt

Abstract:

Toxoplasma gondii infection occurs through ingestion of meat containing cysts, intake of food or water contaminated with cat feces or by handling infected animals. The immunization of animals by an appropriate vaccine could interrupt the circle between animals and humans. The present work is aimed to study the effect of vaccination with radiation-attenuated tachyzoites of Toxoplasma gondii RH strain and compare it with the therapeutic effect of some herbs as curcumin (Curcuma longa) or pomegranate (Punica granatum) as alternative medicine for toxoplasmosis. To assess the cellular immune response the level of TNF-α and IL-10 response were also studied. Tachyzoites were attenuated with both ultraviolet radiation at 2.47 mw-min/cm 2 and gamma radiation at 0.3 KGy. Male mice were infected with 2x10 3 tachyzoites/ml and treated with C. longa and P. granatum at 100 and 200 mg/Kg body weight /day for three successive days. Other groups were injected with attenuated tachyzoites and challenged with 2x10 3 active tachyzoites after three weeks. To compare the efficiency of vaccination and herbal treatment, a group of animals was treated with septrin at dose of 100 and 200 mg/Kg body weight/day. The group vaccinated with UV radiation-attenuated tachyzoites showed better growth inhibition (96.6%) of tachyzoites compared with gamma attenuated group (94.5%). Also, P. granatum showed better growth inhibition at dose 200 mg/Kg (88.9 %) compared to C. longa which gave 82.3% growth inhibition, while septrin showed 88.9% growth inhibition of tachyzoites.

Page(s): 241-248

DOI: DOI not available

Published: Journal: Pakistan Journal of Zoology, Volume: 48, Issue: 1, Year: 2016

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