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Toxic Effects of Aflatoxin B1 on Hematobiochemical and Histopathological Parameters of Juvenile White Leghorn Male Birds and their Amelioration with Vitamin E and Moringa oleifera
Author(s):
1. Muhammad Kashif Saleemi: Department of Pathology, University of Agriculture Faisalabad,,Pakistan
2. Ahmad Raza: Department of Pathology, University of Agriculture Faisalabad,,Pakistan
3. Aisha Khatoon: Department of Pathology, University of Agriculture Faisalabad,,Pakistan
4. Muhammad Zubair: Department of Veterinary Clinical Sciences, University of Poonch,Rawalakot, Azad Kashmir,Pakistan
5. Yongping Xu: School of Bioengineering, Dalian University of Technology, No. 2 Linggong Road, Dalian 10 116024, China;
6. Bilal Murtaza: School of Bioengineering, Dalian University of Technology, No. 2 Linggong Road, Dalian 10 116024, China
7. Xiaoyu Li: School of Bioengineering, Dalian University of Technology,No. 2 Linggong Road, Dalian, China;
8. Maria Jamil: Department of Pathology, University of Agriculture Faisalabad,,Pakistan
9. Muhammad Imran: Department of Pathology, University of Agriculture Faisalabad,,Pakistan
10. Faqir Muhammad: Faculty of Veterinary Sciences, Bahauddin Zakariya University Multan,,Pakistan
11. Khawar Zubair: Department of Pathology, University of Agriculture Faisalabad,,Pakistan
12. Sheraz Ahmed Bhatti: Faculty of Veterinary Sciences, Bahauddin Zakariya University Multan,,Pakistan
13. Muhammad Kamran Rafique: Department of Pathobiology (Pathology Section), University of Veterinary and Animal Sciences, Lahore, Sub Campus Jhang, Pakistan
14. Ishtiaq Ahmed: Department of Pathobiology (Pathology Section), University of Veterinary and Animal Sciences, Lahore, Sub Campus Jhang, Pakistan
15. Farhan Azal: Poultry Research Institute,Shamas Abad Murree Road Rawalpindi,Pakistan
16. Farhat Jubeen: Department of Chemistry, Government College Women University Faisalabad,,Pakistan
17. Abdul Basit: HQ Military Farms, Remount Veterinary and Farm Corps,Okara,Pakistan
Abstract:
Mycotoxins are secondary metabolites produced by pathogenic fungi under suitable environmental conditions. Moringa oleifera and vitamin E are known antioxidants and immunostimulants. In present experimental study, M. oleifera and vitamin E were administered in birds feed to mitigate the toxico-pathological impacts of aflatoxin B1 in white Leghorn layer males. A total of 120 white Leghorn layer male birds were equally divided into six experimental groups. Group A was kept as negative control, group B was fed 400ppb of aflatoxin, while D and F groups were fed normal feed and supplemented with vitamin E (100ppm) and 1 %M. oleifera, respectively. The birds in C and E groups were given 400 ppb AFB1 in feed and supplemented with vitamin E and M. oleifera, respectively. During the 60 days of experimental trial, hematobiochemical and histopathological parameters were studied. The data obtained was analysed by analysis of variance test and group means were compared by DMR test using M STAT C statistical software. During and at the end of experimental trial, male layer birds were slaughtered, collected kidney and liver were fixed in 10% neutral buffered formalin to investigate gross and histopathological alternations. There were significant alterations in hematological and serum biochemical parameters of aflatoxin treated birds. Serum enzymes including ALT, AST and LDH values were significantly increased in group B in contrast to control group A. The histopathological changes in hepatic and renal parenchyma induced by AFB1 were prominent and no such changes were seen in A, D and F groups, while C and E group showed mild necrotic changes which was an indication of ameliorative effects of vitamin E and M. oleifera. It may be concluded that vitamin E and M. oleifera have potential to mitigate toxicity of aflatoxin B1.
Page(s): 405-411
Published: Journal: Pakistan Veterinary Journal, Volume: 43, Issue: 3, Year: 2023
Keywords:
Aflatoxin Vitamin E Moringa oleifera Histopathology Serum chemistry Hematology
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