A Survey for Two Major Grapevine Viruses in Georgian Vineyards, Caucasus Region | [International Journal of Agriculture and Biology • 2022]

Author(s):

1. Tinatin Elbakidze: Plant Pest Diagnostics Department, Laboratory of Molecular Biology, LEPL - State Laboratory of Agriculture,0159, Tbilisi,Georgia; School of Science and Technology, University of Georgia,0171 Tbilisi,Georgia

2. Dali Gaganidze: Plant Pest Diagnostics Department, Laboratory of Molecular Biology, LEPL - State Laboratory of Agriculture,0159, Tbilisi,Georgia

3. Mariam Aznarashvili: Plant Pest Diagnostics Department, Laboratory of Molecular Biology, LEPL - State Laboratory of Agriculture,0159, Tbilisi,Georgia

4. Ketevan Badalashvili: Plant Pest Diagnostics Department, Laboratory of Molecular Biology, LEPL - State Laboratory of Agriculture,0159, Tbilisi,Georgia

Abstract:

This study represents the distribution of Grapevine fleck and G. fanleaf viruses in the vineyards of the Kakheti region, the number one winemaking area in the republic of Georgia and highlights the benefit of the timely identification of phytopathogens using reliable laboratory assays. The main goal of the proposed study was to identify grapevine viruses in two prominent cultivars: Rkatsiteli and Saperavi. A survey was conducted in the 2020-2021 growing seasons. In total 600 grapevine samples were collected from 30 vineyards and analyzed using Double-antibody sandwich enzyme-linked immunosorbent assay (Das-ELISA) and One-Step RT-PCR analysis. Out of 123 positive samples 112 tested positive for G. fleck virus and 11- for G. fanleaf virus respectively based on Das-ELISA assay. Mix infection was demonstrated in 2 tested Saperavi cultivars. G. fleck virus was prevalent and both viruses were predominantly distributed in the Saperavi cultivar. Randomly selected 28 positive and 20 negative samples were further confirmed using a One-Step RT-PCR assay that revealed the effectiveness of Das-ELISA. A comprehensive diagnosis is important for controlling grapevine viruses and helps develop regulatory measures further to establish improved strategies for using virus-free planting materials.

Page(s): 113-117

DOI: 10.17957/IJAB/15.1958

Published: Journal: International Journal of Agriculture and Biology, Volume: 28, Issue: 2, Year: 2022

Keywords:

Vitis vinifera L , Serological assay , Molecular assay , Viral disease , Grapevine viruses

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