Using AFLP to Identify genetic Relationships in Cassia Species from Thailand. | [Pakistan Journal of Botany • 2017]

Author(s):

1. ANUSARA SIHANAT: College of Public Health Sciences, Chulalongkorn University,Bangkok 10330,Thailand

2. PIYARAT PARINYAPONG CHAREONSAP: Plant Genetic Conservation Project under The Royal Initiation of Her Royal Highness Princess Maha Chakri Sirindhorn (RSPG),Chitralada Palace, Dusit, Bangkok 10303,Thailand

3. NIJSIRI RUANGRUNGSI: College of Public Health Sciences, Chulalongkorn University,Bangkok 10330,Thailand: Faculty of Pharmacy, Rangsit University,Pathumthani 12000,Thailand

4. KANCHANA RUNGSIHIRUNRAT: College of Public Health Sciences, Chulalongkorn University,Bangkok 10330,Thailand

Abstract:

Several species of Cassia are used in Thai folk medicine as a laxative and a treatment for skin infections. However, the taxonomy of the GenusCassia is quite complex and intriguing. Thus, the correct identification of the species of this genus is necessary for efficacy and safety. The phylogenetic relationships among the 16 species of Cassiagenus existing in Thailand were evaluated using Amplified Fragment Length Polymorphism (AFLP) technique. Combinations of 70 primers were screened and eleven primer combinations produced a total of 849 distinct and reproducible bands ranging from 60 to 100 bands with an average of 77.18 bands per primer combination. The genetic distances were calculated based on the AFLP bands that had been amplified using the eleven primer combinations. The similarity indices (SI) ranged from 0.25 to 0.78. The dendrogram was created using the Unweighted Pair Group Method of the Arithmetic Average (UPGMA) and the genotypes were divided into two major groups. The results indicate that the phylogenetic relationships are associated with the morphological characterization. In conclusion, an AFLP marker could be an efficient and reliable tool for the identification of a Cassia species.

Page(s): 1339-1344

DOI: DOI not available

Published: Journal: Pakistan Journal of Botany, Volume: 49, Issue: 4, Year: 2017

Keywords:

Cassia species , Amplified fragment length polymorphism , Phylogenetic relationships

References:

References are not available for this document.

Citations

Citations are not available for this document.

Citations

Downloads

Views