Virulence of Metarhizium anisopliae (Hypocreales: Clavicipitaceae) Strains against the Eggs and Larvae of Rhipicephalus sanguineus (Acari: Ixodidae) | [Pakistan Journal of Zoology • 2021]

Author(s):

1. Laura Alejandra Arriola-Mosqueda: Maestría Interinstitucional en Producción Pecuaria, Universidad de Guanajuato,Irapuato, Guanajuato,México

2. Yadira Jiménez-Lara: Departamento de Veterinaria y Zootecnia, Universidad de Guanajuato,Irapuato, Guanajuato,México

3. Eugenia del Carmen Prieto-Avella: Maestría Interinstitucional en Producción Pecuaria, Universidad de Guanajuato,Irapuato, Guanajuato,México

4. Carlos Ricardo Cruz-Vazquez: National Technological Institute of Mexico, Technological Institute El Llano,Aguascalientes, Aguascalientes,Mexico

5. Roberto Montesinos-Matias: Centro Nacional de Referencia de Control Biológico,Tecomán, Colima,México

6. Mauricio Valencia-Posadas: Departamento de Veterinaria y Zootecnia, Universidad de Guanajuato,Irapuato, Guanajuato,México

7. Ana Martha Cruz-Avalos: Universidad Tecnológica del Sureste de Guanajuato,México

8. Jaime Molina-Ochoa: Coordinación General de Investigación, Universidad de Colima, Tecomán, Colima, México; Department of Entomology, University of Nebraska-Lincoln, Lincoln, USA

9. César Andrés Angel-Sahagún: Departamento de Veterinaria y Zootecnia, Universidad de Guanajuato, Irapuato, Guanajuato, México

Abstract:

The aim of this research was to evaluate the virulence of Metarhizium anisopliae on the eggs and larvae of Rhipicephalus sanguineus, as well as the LC50 on R. sanguineus larvae, were determined and the germination (at 18 and 24 h post-inoculation) and radial growth (after 14 days) of four strains of M. anisopliae. We evaluated the pathogenicity on the eggs and larvae of all tested strains of R. sanguineus at day 3 and 7. The LC50 of four strains of M. anisopliae was determined by Probit analysis, and a chisquare test was used to determine the relationship of LC50 with variable radial growth and germination at 18 and 24 h. Germination at 18 h and 24 h ranged from 83.8–92.5% and 89.5–100%, respectively. The average radial growth ranged from 2.3–3.3 mm per day. The pathogenicity results for eggs and larvae showed 100% mortality for both biological stages. The Ma4 strain showed an outstanding LC50 value with 1.78 × 10E6 conidia mL-1. M. anisopliae is effective for controlling R. sanguineus, and that the radial growth characteristics and germination were not correlated with the fungal virulence against R. sanguineus larvae. We can conclude that there are differences between the evaluated strains of M. anisopliae in regard to germination at 18 and 24 h, radial growth and virulence against the larvae of R. sanguineus. There is also no relationship between the biological characteristics and virulence of M. anisopliae against R. microplus larvae.

Page(s): 1149-1152

DOI: 10.17582/journal.pjz/20190827170848

Published: Journal: Pakistan Journal of Zoology, Volume: 53, Issue: 3, Year: 2021

Keywords:

Biological Control , LC50 , Tick , Metarizium anisopliae , Spore viability

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