Using of DNA-barcoding, SCoT and SDS-PAGE protein to assess soma-clonal variation in micro-propagated fig (Ficus carica L.)plant | [Pakistan Journal of Biological Sciences • 2022]

Author(s):

1. Attia O. Attia: Department of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Kingdom of Saudi Arabia;Agricultural Genetic Engineering Research Institute, Agricultural Research Center, 9 Gamaa St., P.O. Box, 12619 Giza, Egypt

2. Ismail A. Ismail: Department of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Kingdom of Saudi Arabia;Agricultural Genetic Engineering Research Institute, Agricultural Research Center, 9 Gamaa St., P.O. Box, 12619 Giza, Egypt

3. El Dessoky S. Dessoky: Department of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Kingdom of Saudi Arabia; Agricultural Genetic Engineering Research Institute, Agricultural Research Center, 9 Gamaa St., P.O. Box, 12619 Giza, Egypt

4. Bandar S. Aljuaid: Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Kingdom of Saudi Arabia

Abstract:

Background and Objective: In vitro propagation of fig (Ficus carica L.) is one of the possible approaches that may be used to maximize the diversity of plant species. The current work was carried out to evaluate genetic stability of micropropagated fig plantlets and to determine the effect of in vitro propagation on genomic content of Saudi fig. Materials and Methods: The start codon-targeted (SCoT), DNA-barcoding chloroplast gene RNA polymerase1 (rpoC1 sequencing) and total protein profiling assays (SDS-PAGE) techniques were used to detect genetic stability in micropropagated fig plantlets. Results: The Scorable PCR bands were produced with 10 SCoT primers used, where the total number of bands was 135 bands. Twenty polymorphic bands were generated with 18.4% of a polymorphism percentage. According to the result, no visual unique bands were generated which confirmed the genetic homogeneity of micropropagated plantlets samples compared to the control sample (mother plant). Sequence analysis and phylogenetic tree generated using fig rpoC1 sequence showed high similarity between control and plantlets samples of fig plant. The protein profiling results revealed no remarkable changes between micropropagated plantlets and the mother plant. Conclusion: The results indicate that using SCoT, DNA barcoding and protein profiling have demonstrated their utility to detect genetic homogeneity in micropropagated fig plantlets, which suggests using of micropropagation protocol of plants applied on the plantlets in the current study as a reliable protocol for in vitro culture and conservation of fig plant.

Page(s): 415-425

DOI: 10.3923/pjbs.2022.415.425

Published: Journal: Pakistan Journal of Biological Sciences, Volume: 25, Issue: 5, Year: 2022

Keywords:

SDSPAGE , Micropropagation , Fig Ficus carica L , somaclonal variation , DNAbarcoding , SCoT

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