Abstract:
A Bacillus sp. MTZ-1 was examined for amylase production along with its substrate specificity, applications, pH, carbon source and NaCI concentration at different time intervals. Maximum enzyme activity was recorded at pH 7 after96 H of incubation while addition of 0.5% NaCI and 1% maltose increased the production of enzyme. Substrate specificity was maximum against malt starch followed by wheat starch, rice starch, corn starch and soluble starch, respectively. Crude enzyme showed fruit juice clarification when introduced into fresh apple juice to remove haze. Enzyme preparations showed antibacterial activity against V ancomyci n-Resistant Enterococci (V RE), Bacilus subtilis, Pseudomonas sp., Acinetobacter sp., Enteropathogenic Escherichia coli (EPEC), M ethi ci I I in-Resistant S. aureus (M RSA), Klebsiella sp., Shigella sp., Staphylococcus epidermiditis, E. coli and S. aureus. Antifungal activity of the crude enzyme also showed inhibition of many fungal pathogens including Aspergillus niger, A. flavus, A. tereus, Penicillium sp., Rhizopus sp., Trichophyton tonsurans and Mucor sp. by Agar well diffusion method.
Page(s):
513-521
DOI:
DOI not available
Published:
Journal: International Journal of Biology and Biotechnology, Volume: 13, Issue: 4, Year: 2016