TLR2 and TLR4 as a Biomarker of Bacterial Sepsis Syndrome in Adult and Children Patients in Iraq. | [Pakistan Journal of Biotechnology • 2018]

Author(s):

1. Farah Q Younis: Department of Biology, College of Science University of Mustansiriya, Iraq

2. Ali Hussein Alwan: Department of Biology, College of Science University of Mustansiriya, Iraq

3. Neihaya H Zaki: Department of Biology, College of Science University of Mustansiriya, Iraq

Abstract:

This study aimed to evaluate the using of Toll-like receptors (TLR2 and TLR4) gene expression as an early biomarkers for diagnosis of bacterial septic syndrome in children and elderly. The causative agents of infection were determined by blood culture. Tumor Necrosis Factor alpha (TNF-a), Interleukin 10 (IL-10), and Soluble Human Leukocyte Antigen - antigen D Related (sHLA-DR) was measured by enzyme-linked immunosorbent assay (ELISA) and TLR2, TLR4 expression was determined by quantitative real-time PCR. We included 75 patients was diagnosed with sepsis syndrome.The age range of patients (13 days-92 years) with mean 56.3±13.9 and matched to 55 healthy volunteers. Depending on age, patients were divided into 4 age groups (group A 40 year). The results indicated that the levels of TNF-a, IL-10 and sHLADR in all age groups were significantly increased (P<0.05) except the level of TNF-a in group B (P=0.123) compared with control groups. A positive correlation has observed between levels of TNF-a and sHLA-DR with patients age (P-value=0.044 and P-value= 0.00013),respectively. TLR2 and TLR4 mRNA expression was significantly increased in all age groups with significant difference between group A and groups D. TLR2 expression highly increased in G+ve infection, while TLR4 expressed highly in G-ve infection. We conclude that TLR2 and TLR4 expression in bacterial sepsis patients indicates the strong possibility of using them as biomarkers in the early diagnosis of bacterial sepsis syndrome, in children and elderly patients

Page(s): 129-134

DOI: DOI not available

Published: Journal: Pakistan Journal of Biotechnology, Volume: 15, Issue: 1, Year: 2018

Keywords:

Keywords are not available for this article.

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