The Role of CSGA and BCSA Genes on Biofilm Formation and Virulence in Salmonella Enterica serovar typhimurium. | [Pakistan Veterinary Journal • 2018]

Author(s):

1. Muhanad El Hag: College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, PR China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, Jiangsu, PR China: Jiangsu Research Centre of Engineering and Technology for Prevention and Control of Poultry Disease; Yangzhou, Jiangsu, 225009, P.R. China

2. Zheng Feng: College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, PR China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, Jiangsu, PR China: Jiangsu Research Centre of Engineering and Technology for Prevention and Control of Poultry Disease; Yangzhou, Jiangsu, 225009, P.R. China

3. Yangyang Su: College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, PR China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, Jiangsu, PR China: Jiangsu Research Centre of Engineering and Technology for Prevention and Control of Poultry Disease; Yangzhou, Jiangsu, 225009, P.R. China

4. Tao Qin: College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, PR China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, Jiangsu, PR China: Jiangsu Research Centre of Engineering and Technology for Prevention and Control of Poultry Disease; Yangzhou, Jiangsu, 225009, P.R. China

5. Sujuan Chen: College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, PR China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, Jiangsu, PR China: Jiangsu Research Centre of Engineering and Technology for Prevention and Control of Poultry Disease; Yangzhou, Jiangsu, 225009, P.R. China

6. Daxin Peng: College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, PR China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, Jiangsu, PR China: Jiangsu Research Centre of Engineering and Technology for Prevention and Control of Poultry Disease; Yangzhou, Jiangsu, 225009, P.R. China

7. Xiufan Liu: College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, PR China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, Jiangsu, PR China: Jiangsu Research Centre of Engineering and Technology for Prevention and Control of Poultry Disease; Yangzhou, Jiangsu, 225009, P.R. China

Abstract:

Biofilm formation can increase bacterial resistance to adverse conditions. However, limited information is available regarding the roles of the csgA and bcsA genes involved in biofilm formation and virulence for Salmonella enterica serovar Typhimurium. Here we deleted the csgA and bcsA genes in S. Typhimurium strains S016 and S025 and assessed several aspects of biofilm formation and virulence. The ?csgA strains did not produce curli fimbriae and ?bcsA mutants had decreased cellulose production. The ?csgA strains were unable to form biofilms. The ?csgA strains also showed decreased adhesion and invasion to HeLa cells and reduced intracellular proliferation in HD11 macrophages. The ?bcsA mutants had similar adhesion, invasion, and proliferation as compared to the wild-type strains. The ?csgA strains were significantly attenuated in the virulence in assays involving oral challenge of one-day-old chickens. These findings clarify the respective roles of csgA and bcsA in biofilm formation and pathogenicity of S. Typhimurium.

Page(s): 159-164

DOI: 10.29261/pakvetj/2018.049

Published: Journal: Pakistan Veterinary Journal, Volume: 38, Issue: 2, Year: 2018

Keywords:

bcsA Biofilm csgA Salmonella Typhimurium Virulence

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