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Development and validation of spectrophotometric method for the detection of azo dyes in feed samples
Author(s):
1. Sadia Nawaz:
2. Rida Fatima:
3. Rahat Naseer:
Abstract:
Azo dyes cause toxic effects on its consumers. The present methods for their identification and quantification are HPLC and UHPLC-MS/MS which are expensive and technological complicated. Here we established a simple method for the detection and quantification of malachite green and methylene blue dyes in feed samples by using a spectrophotometer. The samples were extracted by using acetonitrile and purified by using solid-phase extraction. Dyes spiked in feed were tested in terms of linearity, recovery, repeatability. Recoveries were in the range of 92-97%. The developed method could be used for the determination and quantification of azo dyes in feed samples.
Page(s): 134-134
DOI: DOI not available
Published: Journal: Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 , Volume: 0, Issue: 0, Year: 2023
Keywords:
Spectrophotometer , Validation , Azo dyes , feed samples
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