Abstract:
The study was designed to investigate the effect of essential parameters for the production of Pleurotus ostreatus mother spawn. Firstly, culturing was done on agar media under aseptic conditions in the Applied Botany Lab, Department of Botany, Government College University, Lahore. The optimization of culturing incubation temperature was done at 20°C, 25°C and 30°C temperatures. The maximum growth of mycelium was seen at 25°C. Secondly, Optimization of mother spawn was carried out by employing parameters like carbon source and incubation temperature. The following carbon sources were used like wheat grain, sorghum and pearl millet. Incubation of mother culture was done at 20°C, 25°C and 30°C temperatures. The experiment was performed by using completely randomized design (CRD). At 20°C temperature, pearl millet as a carbon source showed maximum spawn run. Similarly, at 25°C pearl millet again showed more spawn run than other two carbon sources, and on 30°C temperature no growth was revealed by any of the carbon source due to overheating. When spawn run of pearl millet was compared at 20°C and 25°C, it was seen that at 25°C temperature Pleurotus ostreatus showed maximum mycelial growth. Both for culturing and spawning 25°C temperature and pearl millet as carbon source was optimized. This data will be further used for more optimization of mother spawn and fruiting of Pleaurotus ostreatus.
Page(s):
158-158
DOI:
DOI not available
Published:
Journal: Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 , Volume: 0, Issue: 0, Year: 2023