Abstract:
Proximal tubule cell suspension was prepared by proteolytic digestion of the whole chick kidney. The observed insignificant release of lactate dehydrogenase and alkaline phosphatase employed that there were only few broken cells present in the suspension. By microscopic examination, the suspension appeared to have predominant population of cells from the proximal portion of the nephron. The metabolic efficiency of the cells was measured by the production of CO2 from glucose. The cells were shown to be gluconeogenic and have very little glycolytic activity. The preparation therefore, consisted of homogeneous population of viable proximal tubule fragments and can be used in metabolic and transport studies.
Page(s):
21-26
DOI:
DOI not available
Published:
Journal: Journal of Research Science, Volume: 3, Issue: 1, Year: 1991