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Differential gene expression analysis of chloride ion channels in bladder cancer patients of pakistan
Author(s):
1. Muhammad Sheryar: Department of Biosciences, COMSATS University Islamabad, Islamabad Pakistan
2. Asma Amjad: Department of Biosciences, COMSATS University Islamabad, Islamabad Pakistan
3. Rouhullah: Institute of Kidney Disease, Hayatabad Medical Complex, Peshawar, KP, Pakistan
4. Muhammad Jawad Khan: Department of Biosciences, COMSATS University Islamabad, Islamabad Pakistan
Abstract:
Oncogene and tumour suppressor gene mutations cause heterogeneous cancer. About 10 million people die due to cancer annually. Bladder cancer is one of the most common cancers worldwide, predominantly in men. It is reported that ion channels are involved in cell proliferation, differentiation, apoptosis, tumour cell motility, invasion, and metastasis; hence, cancers are channelopathies. This study examined the expression of chloride ion channels ANO1, ANO2,ANO6,CLC3, and CLC4 in bladder cancerby using real time polymerase chain reaction (qPCR). We collected 20 pairs of tumours and normal tissue samples from bladder cancer patients for the expression analysis. We observed significantly (P< 0.05) increased ANO1(3.12 folds), ANO2(10.97 folds), ANO6(35.90 folds), and CLC3(78.37 folds) mRNA expression and a decrease in CLC4(0.74 folds) expression in the tumours. Mutations inANO1, ANO2, ANO6 and CLC3 genes cause chloride ion channel changes that promote cancer. Understanding chloride channel molecular mechanisms require more research.
Page(s): 261-261
DOI: DOI not available
Published: Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
Bladder cancer , ANO1 , ANO3 , ANO2 , Chloride Ion Channels
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