Use of RAPD Markers in Comparison with Agro-morphological Traits for Estimation of Diversity among Chickpea Genotypes. | [International Journal of Agriculture and Biology • 2017]

Author(s):

1. Ahmad Bakhsh: National Agricultural Research Centre, Islamabad, Pakistan

2. Sheikh M Iqbal: Department of Microbiology, University of Haripur, Pakistan

3. Arshad Javaid: Institute of Agricultural Sciences, University of the Punjab Lahore, Pakistan

Abstract:

Genetic diversity was assessed among 38 chickpea (Cicer arietinum L.) genotypes on the basis of random amplified polymorphic DNA (RAPD) in comparison with agro-morphological traits. Evaluation of agro-morphological traits revealed highly significant differences among genotypes. Days to 50% flowering ranged from 92-118, plant height 54.16-87 cm, number of fruit bearing branches 4-17.25, number of pods per plant 7.6-27.4, and grain yield per plant 3.5?9.8 g. Ascochyta blight (caused by Ascochyta rabiei) score of these genotypes was recorded on 1-9 rating scale that varied from 3-9. Cluster analysis showing relationship based on morphological traits (scale: Euclidean distance) placed 35 genotypes into five distinct groups, while three genotypes namely Noor-91, Local Mankera and BR4 did not include in any cluster. RAPD analysis showed that 35 RAPD primers amplified a total of 212 fragments out of which 45 were polymorphic. Polymorphic bands were generated by 21 primers whereas 14 primers were monomorphic. Genetic similarity matrix based on Nei and Li's index revealed similarity coefficients ranging from 92-97% indicating lower level of genetic polymorphism revealed by RAPD primers. Dandrogram constructed on the basis of these coefficients grouped all the genotypes into 2 major and 3 small clusters at 92% similarity level. Two decamers, OPC5 and OPC14 distinguished between three Desi and two Kabuli genotypes. This study showed that the level of genetic variability observed in chickpea for agro-morphological traits was not reflected in DNA polymorphism obtained by RAPD analysis.

Page(s): 427-431

DOI: 10.17957/IJAB/15.0293

Published: Journal: International Journal of Agriculture and Biology, Volume: 19, Issue: 3, Year: 2017

Keywords:

Keywords are not available for this article.

References:

[1] Agrama , H.A.,M.R.Tuinstra, 2004.Phylogenetic diversity and relationships among sorghum accession using SSRs and RAPDs, 2 334 -340

[2] Alam , A.R.,M.A.Habib,S.S.Alam, 2012.Karyotype and RAPD analysis in five potato varieties (Solanum tuberosum L.),Bangl. J. Bot. 41 105 -110

[3] Ayana , A.,T.Bryngelsson,E.Bekele, 2000.Genetic variation of Ethopian and Eritrean sorghum (Sorghum bicolour) germplasm assessed by random amplified polymorphic DNA (RAPD),Gene. Res. Crop Evol 47 471 -482

[4] Bakhsh , A.,T.Gull,A.Qureshi,M.Arshad,B.A.Malik, 1999.Genetic variability and character correlation in pure lines, F1 and F2 progenies of chickpea (Cicer arietimum L.),Pak. J. Bot. 31 41 -53

[5] Chowdhary , M.A.,B.Vandenberg,T.Warkentin, 2002.Cultivar identification and genetic relationship among selected breeding lines and cultivars in chickpea (Cicer arietinum),Euphytica 127 317 -325

[6] Collard , B.C.Y.,E.C.K.Pang,P.W.J.Taylor, 2003.Selection of wild Cicer accessions for the generation of mapping population segregating for resistance to ascochyta blight,Euphytica 130 1 -9

[7] Dey , R.C.,L.Hassan,L.A.Z.Al-Munsur, 2012.Analysis of genetic variability in regenerated and germinated plantlets of ten Indica rice genotypes (Oryza sativa L.) using RAPD markers,Agronomica 56 55 -64

[8] Doyle , J.J.,J.L.Doyle, 1990.Isolation of plant DNA from fresh tissue,Focus 12 13 -15

[9] FAOSTAT, 2012.,on February 7 2015 -

[10] Iruela , M.,J.Cubero,J.I.Cubero,J.Gill,T.Millan, 2002.Phylogenetic relationship in the genus Cicer cultivated chickpea using RAPD and ISSR markers,Theor. Appl. Genet 104 643 -651

[11] Kafeel , A., 2014.RAPD and SSR based genetic diversity analysis of elite-2 set of synthetic hexaploid wheats,Afr. J. Tradit. Complem. 11 9 -13

[12] Micheli , M.R.,R.Bova,E.Pascale,E.D.Ambrosio, 1994.Reproducible DNA fingerprinting with the Random Amplified Polymorphic DNA (RAPD) method,Nucleic Acids Res 22 1921 -1922

[13] Nei , M.,W.H.Li, 1979.Mathematical model for studying genetic variation in terms of restriction endonucleases,Proc. Natl. Acad. Sci. USA 76 5269 -5273

[14] Sant , V.J.,A.G.Patankar,N.D.Sarode,L.B.Mhase,M.N.Sainani,R.B.Deshmukh,P.K.Ranjekar,V.S.Gupta, 1999.Potential of DNA markers in detecting divergence and analysing heterosis in Indian elite chickpea cultivars,Theor. Appl. Genet 98 1217 -1225

[15] Singh , K.,G.C.Hawtin,Y.L.Nene,M.V.Reddy, 1981.Resistance in chickpeas to Ascochyta blight,Plant Dis 65 586 -587

[16] Shaukat , A.,A.Bakhsh,M.Anwer,A.M.Haqqani, 2002.Exploitation of genetic variability for grain yield improvement in chickpea,Int. J. Agric. Biol 4 148 -149

[17] Simon , C.J.and F.J.,Muehlbauer, 1997.Construction of chickpea linkage map and its comparison with map of pea and lentil,J. Hered. 88 115 -119

[18] Sonnante , G.,A.Marangi,D.Pignon,G.Venora, 1997.Using RAPD markers to investigate genetic variation in chickpea (Cicer arietinum L),J. Genet 51 303 -307

[19] Winter , P.,S.M.Udupa,B.Huttel,P.C.Sharma,S.Sahi,E.R.Arreguine,F.Weigand,F.J.Muehlbaur,G.Kahl, 1999.Characterisation and mapping of microsatellite sites in chickpea (Cicer arietinum L.) genome,Mol. Gen. Genet 262 90 -101

[20] 2017.,(Received 15 August 2016; Accepted 11 February -

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