Abstract:
Worldwide legumes are being cultivated and grown agriculturally not only for human consumption but animals as well. In tropical areas of Asia legume are being used as staple food. One of the important legumes is Mash (black gram) which can easily be grown in the marginal land in Pakistan. The crop is prone to different challenges including nutritional imbalance, soil pH and pathogens which deteriorate crop quality and limit its yield. Despite of its nitrogen fixing ability, fungal/and bacterial pathogen are affecting the nutritional and pharmaceutical properties of the Mash. In this study we focused on PGPRs and more specifically rhizobacteria to analyze and comprehend their ability to cope with phytopathogens as well as providing legumes support by making nutrients available to them. For this purpose different assays were performed. characterized strains on the basis of morphology such as growth, size margins, opacity, elevation etc. Initially PGPRs were isolated from root nodules as well as rhizosphere of black gram on LB agar plates. Then gram negative PGPRs were confirmed through gram staining out of total 41 isolated strains. In our study strain AM-III, AMIV and NCS Red-S showed significant antagonistic activity against fungus whereas, the results of phosphorus solubilization depicted that out of 41 strains 24 strains tested positive for phosphorus solubilization. Out of 41 strains AM-IV, M97 Red-L, M97 Unique 1 and 2 demonstrated most significant results of all for phosphorous solubilizing. In case of AM-V, M97-White, NCS-2 Purple, AND NARC-Yellow 1 excellent zinc solubilization activity was observed whereas, no zinc solubilization activity was to be seen in NM-2, NM-3, M3-2, MASH-III etc. From all this data we can screen out the most affective PGPRs/rhizobacteria and use them as bio fertilizers in future which could help the world to overcome food shortage.
Page(s):
262-262
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
Pathogens
,
PGPR
,
Legumes
,
biofertilizers
,
Agriculture