Abstract:
Amongst naturally available biopolymers lignin came at second position, comprised of asymmetrically assorted phenyl polymers. Lignin degradation significantly important for photosynthetically fixed carbon recycling. In present research, potential fungal strains were isolated from paper mill effluent (black liquor) in order to investigate its probability for the biodegradation of lignin. The four fungus strains (M1, M2, M3, M4) were screened on the basis qualitative and quantitative assays using black liquor and Kraft lignin. The nanozyme approach has been used to mimic the enzyme activity by nanoparticles to enhance lignin degradation ability of the strains. The results of initial screening indicated that the selected strains showed ligninolytic zone on MSN agar plates supplemented with black liquor (BL). The molecular identification suggest that the selected strains are Aspergillus terreus, Dipodasceus australiansis, Geotrichum candidum and Aspergillus fumigatus and belongs to ascomycetes. For insoluble lignin residues all the four selected strains observed a clear growth. Under optimized conditions Aspergillus terreus (M1) shows 73%, Dipodasceus australiansis (M2), Geotrichum candidum (M3) shows 84% and Aspergillus fumigatus (M4) 80% lignin degradation respectively. The biodegradation efficiency was consolidated with the help of secretome and FTIR analysis which indicated transformation of complex structure of lignin. Our results suggested that the eco-friendly approach of nanoparticles with selected ascomycetes strains possess excellent lignin degradation capability and can be used effectively in commercial waste treatment system for pulp and paper mill effluent.
Page(s):
0-0
DOI:
DOI not available
Published:
Journal: First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book), Volume: 0, Issue: 0, Year: 2022
Keywords:
process optimization
,
Lignin degradation
,
Black liquor
,
Nanoparticles