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The Role of Hla-drb1 Allele in Hypothyroid Patients with and without Periodontitis.
Author(s):
1. Sahar H AlHindawi: Department of Basic Science, Physiology, College of Dentistry, University of Baghdad, Microbiology, Iraq
2. Batool H Al-Ghurabi: Department of Basic Science, Microbiology, College of Dentistry, University of Baghdad, Iraq
3. Noori M Luaibi: Department of Biology, College of Science, Al- Mustansiriyah University, Iraq
Abstract:
Hypothyroidism is a frequent disorder in the general population, especially among women, is defined as a deficiency of thyroid activity that results from insufficient production or action of thyroid hormones leading to a total decrease of metabolic. Human leukocyte antigen is the most polymorphic genetic system in man. Genes of this region influence susceptibility to certain diseases. Objectives: The purpose of the present study is to investigate the role of HLA-DRB1 genotyping in hypothyroid patients with and without periodontitis. Sixty hypothyroid patients 30 of patients were with periodontitis and 30 without periodontitis compare with 30 healthy subjects as control enrolled in this study. DNA was extracted from blood samples, then HLA- genotyping performed by polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSO). The results showed the frequencies of HLA-DRB1*03 and *04 alleles are significantly increased in hypothyroid patients than control (P<0.001; P<0.05) respectively; whereas, the frequencies of HLA-DRB1*08 allele is significantly higher in control group as compared to patients group (P<0.05). Surprisingly comparison between groups of hypothyroid patients with and without periodontitis revealed significant higher frequency (P<0.05) of DRB1*03 allele among group of hypothyroid patients with periodontitis. The present findings suggested that the presence of HLA-DRB1*03 and HLA-DRB1*04 alleles may increase the susceptibility to hypothyroidism, while HLA-DRB1*08 allele could confer protective effects against this disease. Moreover, patients with HLA-DRB1*03 allele are more likely to develop periodontitis.
Page(s): 629-634
DOI: DOI not available
Published: Journal: Pakistan Journal of Biotechnology, Volume: 14, Issue: 4, Year: 2017
Keywords:
Keywords are not available for this article.
References:
[1] Ahn , S.,H.B.Choi, 2011.,HLA and Disease Associations in Koreans. Immune Netw 11 324 -335
[2] Al-Sabary , A. H.,M. S.Abdul Razzaq, 2017.Genotyping of Helicobacter Pylori isolated from Iraqi patient with upper gastrointestinal disease,Journal of Global Pharma Technology 9 121 -127
[3] Babu , N.S.,P.B.Patel, 2016.Oral health status of children suffering from thyroid disorders,J Indian Soc of Pedo Preventive Dent 34 139 -144
[4] Bottazzo , G.F.,R.P.Borrell,T.Hanafusa,M., 1983.Feld - mann, Role of aberrant HLA-DR expression and antigen presentation in induction of endocrine autoimmunity,Lancet 2 1115 -1119
[5] Boughman , J. A.,S.L.Halloran,D.Roulston,S.Schwartz,J.B.Suzuki,L.R.Weitkamp,R.E.Wenk,R.Wooten,M.M.Cohen, 1986.An autosomal-dominant form of juvenile periodontitis: Its localization to chromosome 4 and linkage to dentinogenesis imperfecta and Gc,J. Craniofac. Genet. Dev. Biol 6 341 -350
[6] Bougham , J.A.,J.A.Astemborski,M.G.Blitzer, 1990.Early onset periodontal disease: A genetics perspective,Crit. Rev. Oral Biol Med 1 89 -99
[7] Chandna , S.,M.Bathla, 2011.Oral manifestations of thyroid disorders and its management,Indian J. Endocrinol. Metab 15 S113 -116
[8] Chatterjee , A,S.K.Sinha,G.Mukherjee,A Comprehensive, 2014.Review on HLA and its Detections by Polymerase Chain Reaction Technique: Review Article,IJPRS 3 340 -346
[9] Cho , W.K.,M.H.Jung,E.J.Choi,H.B.Choi,T.G.Kim,B.K.Suh, 2011.Association of HLA alleles with autoimmune thyroid disease in Korean children,Horm. Res. Paediatr 76 328 -334
[10] DeRuiter , J., 2002.,Thyroid Hormone Tutorial: Thyroid Pathology. Endocrine Module-Thyroid Section 1 -29
[11] Ersoy , R.,I.Yetkin,S.Gonen,O.Soylemezoglu,E.Akbay,M.Arslan, 2007.04 and HLA-DQB1*0201 Association with Chronic Autoimmune Thyroiditis in a Turkish Population,Turk Jem 11 29 -33
[12] Faas , S.,M.Trucco, 1996.The genes influencing the susceptibility to IDDM in humans,J. Endocrinol. Invest 17 477 -495
[13] Gomes , F.I.F.,M.G.B. Aragão , F.C.B.,Barbosa,M.M. Bezerra , V.P.T.,Pinto and H.V.,Chaves, 2016.Inflammatory Cytokines Interleukin-1β and Tumour Necrosis Factor-α - Novel Biomarkers for the Detection of Periodontal Diseases: a Literature Review,J. Oral Maxillofac. Res 7 1 -10
[14] Gowda , M., S.,Ambardar , N.,Dighe , A.,Manjunath , C.,Shankaralingu , P.,Hirannaiah , J.,Harting , S.,Ranade , L.,Jagannathan and S.,Krishna, 2016.Comparative Analyses of Low, Medium and HighResolution HLA Typing Technologies for Human Populations,J. Clin. Cell Immunol 399 -
[15] Hanafusa , T.,R.P.Borrell,L.Chiovato,R.C.Russell,D.Doniach,G.F.Bottazzo, 1983.Aberrant expression of HLA-DR antigen on thyrocytes in Graves' disease: relevance for autoimmunity,Lancet 2 1111 -1115
[16] Hart , T.C., 1994.Genetic considerations of risk in human periodontal disease,Curr. Opin. Periodontol 3 11 -
[17] Hasan , B.F.,N. AlHudak,I.D.Abd, 2016.Estimation of thyroid hormones and liver enzymes in hypo and hyperthyroidism in Iraqi women,Int. J. Pharm. Bio. Sci. 7 B707 -713
[18] Hugh , H.,J.R.L.Fudenberg,P.An-Chuan Wang,G.B.Ferrara,BasicImmunogenetics, 1994., -
[19] Hunt , P.J., S.E.,Marshall , A.P.,Weetman , M.,Bunce , J.I.,Bell , J.A.,Wass and K.I. Welsh, 2001.Histocompatibility leucocyte antigens and closely linked immunomodulatory genes in autoimmune thyroid disease,Clin. Endocrinol 55 491 -499
[20] Jacobson , E.M.,A.Huber,Y.Tomer, 2008.The HLA gene complex in Thyroid autoimmunity: from epidemiology to etiology,J. Autoimmun 30 58 -62
[21] Jayan , A.,N.Gautam,R.K.Dubey,Y.Neupane,P.Padmavathi,S.K.Jha,A.K.Sinha, 2015.Prevalence and impact of thyroid disorders based on TSH level among patients visiting tertiary care hospital of South Western Nepal,Nepal. Med 17 6 -10
[22] Jonklaas , J.,A.C.Bianco,A.J.Bauer,K.D.Burman,A.R.Cappola,F.S.Celi,D.S.Cooper,B. W.Kim,R. P.Peeters,M.S.Rosenthal,A. M.Sawka, 2014.Guidelines for the Treatment of Hypothyroidism, 24 1670 -1751
[23] Kindt , T.,R.Goldsby,B.Osborne, 2007.Tolerance and autoimmunity, 408 -110
[24] Kornman , K.S., 2001.Patients are not equally susceptible to periodontitis: Does this change dental practice and the dental curriculum,J. Dent. Educ 65 777 -784
[25] Li , H.,X.Yuan,J.Zhou,C.Li,P.Yang,L.Bu,M., 2014.Zhang and S. Qu, Clinical Evaluation of Various Thyroid Hormones on Thyroid Function,Int. J. Endocrinol. Pp. 1-5 -
[26] Mohssen , S.M.,B.H.Al-Ghurabei,H.K.Al-Juboori,A.Kadem, 2013.Frequency of HLA_ DRB1 Alleles in Iraqi Patients with Chronic periodontitis,Int. J. Immunol 3 25 -28
[27] Patiroğlu , T. A.H.,Akar , A.,Akay and E.,EnginOk, 2015.Hashimoto Tiroidit Hastalarında HLA-DRB1 Alellerinin Dağılımı,Tip Bülteni 49 255 -259
[28] Petrone , A.,G.Giorgi,C.A.Mesturino,M.Capizzi,I. Cascino,L.Nistico,J.Osborn,U. DiMario,R.Buzzetti, 2001.0301 haplotype and lack of association of two polymorphic sites at CTLA4 gene with Hashimoto's thyroiditis in an Italian population,Thyroid 11 171 -175
[29] Pinto , A.,M.Glick, 2002.Management of patients with thyroid disease Oral health considerations,J.Am.Dent.Assoc 133 849 -858
[30] Price , P.,R.Allcock,D.Sayer,M.Garlepp,C.C.Kok,M.French,S.Mallal,F.Chris, 1999.-tiansen, The genetic basis for the association of the 8.1 ancestral haplotype (A1, B8, DR3) with multiple immunopathological diseases,Immunol. Rev 167 257 -274
[31] Pushparani , D.S., 2015.,Role of Cytokines in Periodontal Wound Healing Process - A Review. Pharm. Anal.Chem 1000106 -
[32] Rekha , P.L.,V.Valluri,S.S.Rakh,V.Pantula,M. ISHAQ, 2007.Association of HLA DQ B1* and HLA DR B1* Alleles with Goitrous Juvenile Autoimmune Hypothyroidism-A Case Control Study,J.Clin.Immunol 27 486 -489
[33] Rowe , M.,R.Hoermann,P.Warmingham, 2016.Review of the Diagnosis and Treatment of Hypothyroidism: A Patient's Perspective,Diagos. Treat. Hypo 1 1 -38
[34] Sawai , Y.,L.J. DeGroot, 2000.Binding of human thyrotropin receptor peptides to a Graves' disease predis-posing human leukocyte antigen class II molecule,J. Clin. Endocrinol. Metab 85 1176 -1179
[35] Senthilkumaran1 , S.,V.Sathyaprakash,A.Sundhararajan,A, 2015.Study on Prevalence and Distribution of Subclinical Hypothyroidism in Rural Women,Sch. J. Appl. Med. Sci 3 287 -290
[36] Shiina , T.,K.Hosomichi,H.Inoko,J.K.Kulski, 2009.The HLA genomic loci map: expression, interaction, diversity and disease,J. Hum. Genet 54 15 -39
[37] Sippert , E.A.,C.O.Silva,C.M.Ayo,S.B.Marques,J.E.Visentainer,A.M. Ana, 2015.Maria Sell, HLA Haplotypes and Genotypes Frequencies in Brazilian Chronic Periodontitis Patients, 1 8 -
[38] Soomro , N.S.,I.A.Khan, 2016.Effect of phytohormones on shoot and root regeneration in rose under in vitro conditions,Pak. J. Biotechnol 13 199 -203
[39] Sorlie , D.E., 1995.Medical biostatistics and epidemiology: Examination and Board review 1st ed, 47 -88
[40] Trowsdale , J.,J.C.Knight, 2013.,Complex Genomics and Human Disease. Annu. Rev. Genomics Hum.Genet 14 301 -323
[41] Ueda , S.,D.Oryoji,K.Yamamoto,J.Y.Noh,K.Okamura,M.Noda,K.Kashiwase,Y.Kosuga,K.Sekiya,K.Inoue,H.Yamada,A.Oyamada,Y.Nishimura,Y.Yoshikai,K.Ito,T.Sasazuki, 2014.Identification of independent susceptible and protective HLA alleles in Japanese autoimmune thyroid disease and their epistasis,J. Clin. Endocrinol.Metab 99 E379 -383
[42] Zantut-Wittmann , D.E.,L.Persoli,M.A.Tambascia,E.Fischer,D.F.Maldonado,A.M.Costa,E.J.Pavin,HLA-DQB1, 2004.03 association with the atrophic but not with goitrous form of chronic autoimmune thyroiditis in a Brazilian population,Hormon. Metab. Res 36 492 -500
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