A protocol for DNA extraction from feces of the wild pygmy lorises, Nycticebus pygmaeus. | [Pakistan Journal of Zoology • 2013]

Author(s):

1. Lin Zhang: School of Life Sciences, Yunnan Normal University, Kunming, People’s Republic of China; Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, People’s Republic of China

2. Jinhong Cai: School of Life Sciences, Yunnan Normal University, Kunming, People’s Republic of China

3. Caihong Xiao: School of Life Sciences, Yunnan Normal University, Kunming, People’s Republic of China

4. Zhengkun Wang: School of Life Sciences, Yunnan Normal University, Kunming, People’s Republic of China

Abstract:

We developed a new method to isolate DNA from wild pygmy lorises’ feces. The feces were washedtwo or three times with precooled acetone, which removed numerous potential PCR inhibitors. The DNA was purified with phenol/chloroform after incubation with proteinase K. D-loop region and cytochrome b were amplified and sequenced successfully from the extracted DNA. Comparison with the PCR products demonstrated the fecal DNA extracted by the improved protocol was better than the fecal DNA extracted without acetone preprocessing.

Page(s): 1235-1239

DOI: DOI not available

Published: Journal: Pakistan Journal of Zoology, Volume: 45, Issue: 5, Year: 2013

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