Abstract:
The effect of two chemical mutagens, Sodium Azide (NaN3) and Ethyl Methane Sulphonate (EMS) on germination, MI seedling growth and epicotyl length of soybean (Glycine max L. Merill) was studied. Seeds of the variety Kalatur we5e treated with 10 , 5 x 10-, 10-3, 5 x 10- and 10- M concentrations of NaN3 and 0.2, 0.4, 0.6 and 0.8% solutions of EMS. Germination, seedling height and epicotyl length were affected when higher concentrations of 5 x 10-3 to 10-2 M NaN3 were used, while germination was not affected in the wet sowing of EMS treated seed as compared to dry sowing where a decrease of 23.80% was observed. There was no significant effect of EMS concentrations on seedling height and epicotyl length when the seeds were sown as wet while after dryback sowing both the parameters were decreased considerably at higher doses. The Azide concentration in the range of 103- to 5 x 10-3 M may prove suitable for large scale seed treatment whereas a wet treatment of EMS aiming at on epicotyl length reduction of 20-30% may be chosen for getting high yield of mutations.
Page(s):
169-174
DOI:
DOI not available
Published:
Journal: Sarhad Journal of Agriculture, Volume: 1, Issue: 1, Year: 1985