A Biotechnological Assessment of Aspergillus Niger -Produced Pectinase Enzymes' Antibacterial Activity | [Journal of Pioneering Medical Sciences • 2025]

Author(s):

1. Ammar Adnan Tuama: Science Department, College of Basic Education, University of Diyala, Diyala, 32000, Iraq

2. Sundus Adil Naji: Science Department, College of Basic Education, University of Diyala, Diyala, 32000, Iraq

3. Mohammed Ali Hussein: Science Department, College of Basic Education, University of Diyala, Diyala, 32000, Iraq

4. Nazira Karamova: Department of Microbiology, Institute of Fundamental Medicine and Biology, Kazan (Volga region) Federal University, Kremlevskaya str., 18, Kazan, 420008, Russia

Abstract:

In this study, Aspergillus niger was used for the production of pectinase enzymes under submerged fermentation conditions, aiming to evaluate their potential antibacterial activity. Three fungal isolates were tested and optimal conditions for enzyme production were determined regarding incubation time, temperature and pH. The highest enzymatic activity was observed at 40°C and pH 8 after 48 hours of incubation. Quantitative and qualitative assays revealed that pectinase activity ranged between 1.1 and 4.0 U/mg protein, depending on the carbon and nitrogen sources in the growth medium. The antibacterial activity of the produced pectinase was evaluated using the agar well diffusion method against four bacterial strains: Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and Staphylococcus epidermidis, at concentrations ranging from 4 to 64 µg/mL. The results showed dose-dependent inhibition, with K. pneumoniae showing the highest sensitivity (27.33 mm), followed by E. coli (19.33 mm), S. aureus (15.67 mm) and S. epidermidis (6.33 mm). The study highlights the potential of pectinase enzymes from Aspergillus niger for antibacterial properties, making them valuable biotechnological agents in medical and industrial applications. These enzymes could be used as natural bio-preservatives or therapeutic agents against resistant bacterial strains. Future research should focus on understanding antibacterial mechanisms, optimizing production and evaluating efficacy in real-world systems.

Page(s): 150-155

DOI: 10.47310/jpms2025140522

Published: Journal: Journal of Pioneering Medical Sciences, Volume: 14, Issue: 5, Year: 2025

Keywords:

Fungal fermentation , Aspergillus niger , Antibacterial activity , Pectinase , Enzyme Biotechnology

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