Validation of Functioning Resistant Genes against Malaysian Biotype of Brown Planthopper in Rice Variety, Rathu Heenati | [International Journal of Agriculture and Biology • 2022]

Author(s):

1. Mohamad Bahagia AB Ghaffar: Industrial Crop Research Centre, MARDI Seberang Perai, 13200 Kepala Batas, Pulau Pinang, Malaysia

2. Ruziah Mohd Yusuf: Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

3. Maisarah Mohamad Saad: Paddy and Rice Research Centre, MARDI Seberang Perai, 13200 Kepala Batas, Pulau Pinang, Malaysia

4. Muhammad Fairuz Mohd Yusof: Biotechnology & Nanotechnology Research Centre, MARDI Headquarters, 43400, Serdang, Selangor

5. Shahril Ab Razak: Biotechnology & Nanotechnology Research Centre, MARDI Headquarters, 43400, Serdang, Selangor

6. Habibuddin Hashim: Agric Inov Resources. 08000 Sg Petani, Kedah, Malaysia

7. Noor Azmi Shaharuddin: Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

Abstract:

Rathu Heenati is a donor parent commonly used in the development of resistant rice varieties against brown planthopper (BPH), Nilaparvata lugens, which is a devastating insect pest of rice. The variety is reported to have a broad-spectrum resistance against BPH, through the action of multiple genes and quantitative trait loci (QTL) including Bph3, Qbph3, Qbph4 and Qbph10. The present study was carried out to validate the effectiveness of those genes against Malaysian BPH population. The study was conducted using an F2 segregating population which was obtained from a cross between Rathu Heenati and a Malaysian commercial variety, MR219. Plant damage score was used to estimate the degree of plant tolerance, while the amount of honeydew excretion was used to measure the level of antibiosis. The gene presence in an individual plant was determined based on the segregation pattern of the flanking microsatellite markers of the respective reported genes. Results confirmed the role of Bph3 and the three other QTLs in conferring resistance against the Malaysian biotype of BPH. Marker assisted breeding can facilitate the monitoring of the introgressed genes in the plants of a breeding population.

Page(s): 34-42

DOI: 10.17957/IJAB/15.1896

Published: Journal: International Journal of Agriculture and Biology, Volume: 27, Issue: 1, Year: 2022

Keywords:

microsatellite markers , resistance gene , Quantitative trait locus , Brown plant hopper BPH , Oryza sativa L

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