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Expression Profile of Acid Phosphatase under Salinity Stress to Evaluating the Role of Soluble Phosphorous (Pi)
Author(s):
1. Asma Yaqoob: Department of Biochemistry, Institute of Biochemistry, Biotechnology and Bioinformatics (IBBB), The Islamia University of Bahawalpur, Pakistan (IUB)
2. Faiz ul Hasan Nasim: Department of Biochemistry, Institute of Biochemistry, Biotechnology and Bioinformatics (IBBB), The Islamia University of Bahawalpur, Pakistan (IUB)
Abstract:
Biotic and abiotic stress affects plant physiology by changing whole metabolism. In response to these stresses plant produce signals that triggers cellular and molecular mechanism. Salt stress is one of the major abiotic stresses that is involve in inhibition of plant growth. 20 to 30% agriculture land is affected by salinity worldwide. The present work was done by keeping in view the question that soluble phosphorous (Pi) also contribute resistance under stress by preserving a certain level of inorganic phosphate. In the present study C. ciliaris was grown hydroponically and different concentrations of NaCl i.e., 50mM, 100 mM, 200 mM, 300mM, 350mM, 400mM was applied to check the effect of salinity stress on metabolic enzyme acid phosphatase. For this study spectrophotometric method was used to quantify enzyme activity (relative as well as specific) and native PAGE was done to check the expression of different isotypes of Apase at the level of translation. Interestingly result shows the expression of five different isotypes of acid phosphatase under applied stress that shows not only the antioxidant enzyme but metabolic enzyme also helps the plant to cope stress. Expression of different isoforms of the metabolic enzymes acid phosphatase shows the importance of this enzyme in plant cells in maintaining a certain level of inorganic phosphate to regulate metabolic adjustments. Result proves the beauty of nature to develop an incredible ability in plants in the form of adaptive mechanisms.
Page(s): 62-62
DOI: DOI not available
Published: Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023
Keywords:
Salinity stress , Acid Phosphatase , Soluble Phosphorous Pi
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