Purification and properties of milk-clotting enzyme from Bacillus licheniformis RT7P1 | [Abstract Book on International Conference on Life Sciences (ICLS-23) 11-12 May 22-23 • 2023]

Author(s):

1. Muhammad Sibtain: Gomal University D.I. Khan ,Pakistan

2. Habib Ur Rahman: Gomal University D.I. Khan ,Pakistan

3. Romana Tabassum: National Institute for Biotechnology and Genetic Engineering Faisalabad,Pakistan

4. Sadaf Javaria: Gomal University D.I. Khan ,Pakistan

5. Malik Muhammad Hashim: Gomal University D.I. Khan ,Pakistan

6. Aarif M. Khan: College of Agriculture University of Sargodha,Pakistan

7. Alin Zaman: Gomal University D.I. Khan ,Pakistan

8. Adnan Amin: Gomal University D.I. Khan ,Pakistan

Abstract:

Milk-clotting enzyme production by Bacillus licheniformis RT7P1 was used to study proteolytic and milk-clotting activities of the enzyme using increasing concentrations of skim milk in Basal salt medium by submerged fermentation. The proteolytic and milk-clotting activities were obtained after 120 hours of fermentation with 100% composition of skim milk to the medium. Purified milk-clotting enzyme was obtained by ammonium sulphate precipitation followed by dialysis, DEAE-Cellulose A-50 column and Sephadex G-250 Gel filtration chromatography with 1.25 purification fold and a specific activity of 2.45 U/mg. The optimum proteolytic and milk-clotting activities were at pH 10 and at temperature of 50°C. The metallic ions SrCl2, MnSO4, MnCl2, KCl, NiCl2, FeSO4, increased the PA and MCA of enzyme with increasing concentrations upto 15mM, while MgCl2 increase the activity with 5 mM while decreased the activity when used in higher concentrations. The proteolytic and milk-clotting activities of the enzyme were inhibited by SDS and EDTA. CaCl2 with increasing concentrations from 0.001 to 0.004 mM concentrations increased the milk-clotting activity of the enzyme.

Page(s): 103-103

DOI: DOI not available

Published: Journal: Abstract Book on International Conference on Life Sciences (ICLS-23) 11-12 May 22-23, Volume: 0, Issue: 0, Year: 2023

Keywords:

Bacillus licheniformis

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