Fagonia indica Burm f. (Mushikka or white spine) is a plant distributed in the deserts of Asia and Africa and reported to be medicinal in the scientific literature as well as in the folk medicine. Earlier investigations, the authors isolated a number of bioactive constituents from the plant including flavonoids, sterols and tritepenoids; In addition its flavonoidal content was found remarkably high reaching 3% (calculated as flavonol on dry weight). The present study is an attempt to formulate, characterize and evaluate a natural wound-healing gel preparation containing the crude plant extract. Three formulae (F1-F3) were prepared. The gel properties such as viscosity, swelling ratio, bio-adhesion, in vitro release, stability, microbiological studies, in vivo burn healing test on rats and histopathological features were assessed.The results of the in vitro evaluation and stability studies showed that F3 (0.5% (w/w) of plant extract in 4% (w/w) chitosan) was significantly (pin vivo burn healing and histological results, F3 enhanced the skin wound re-epithelialization and speed up the healing process compared to the conventional commercial product. Thus, the Fagonia extract loaded chitosan topical gel would be used successfully in burn wound care.