Abstract:
Gene therapy offers a highly promising approach to effectively address a range of diseases, including lung cancer. Non viral vectors, such as nanocarriers, have emerged as attractive alternatives to viral carriers due to their simplicity of modification and reduced immunogenicity. However, the low transfection effectiveness of gene therapy is a substantial obstacle that must be overcome. Understanding the endocytic routes used by nanocarriers and their various functions in the transfection process is crucial to overcoming this challenge. The development of more effective gene delivery devices depends on this understanding. The internalization and intracellular trafficking of gene delivery systems are governed by the endocytic pathway, which has a significant impact on how well these systems deliver therapeutic genes to target cells. Gene therapy can be successful by improving transfection efficiency and endocytic process understanding. In a recent work, we sought to evaluate the proportional contributions of the several endocytic pathways used by commercially available nanocarriers in lung cancer-derived cells. We will create H1299 cell cultures derived from human lung cancer, identify and validate a reporter gene, and create nanocarrier complexes using Lipofectamine 2000 and Linear Polyethyleneimine. We intend to clarify the role of each endocytic pathway in the cellular uptake and intracellular trafficking of gene delivery systems by using particular inhibitors to disrupt each pathway while transfecting H1299 cells. Fluorescence microscopy and ImageJ software were used for this investigation, which will give important insights into the most important endocytic routes and direct the development of tactics to increase transfection effectiveness for better gene therapy outcomes. A thorough analysis of nanoparticle absorption through the endocytic route has the potential to make significant advancements in the domains of cell biology and nanomedicine. The results of this work will open the door for novel strategies in the creation and improvement of nanoparticle-based treatments, enhancing the treatment of many diseases and enabling us to fully realise the potential of gene therapy. We want to revolutionise healthcare, enhance patient outcomes, and usher in a new era of precision medicine by figuring out the complex interactions between nanoparticles and cells.
Page(s):
63-63
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
Lung
,
Gene Therapy
,
nanocarriers
,
CancerDerived Cells
,
Endocytic Pathways
,
Transfection Efficiency