Abstract:
Cytokinin (CKs) are classical phyto hormones regulating diverse aspects of plantgrowth and development. Their perception in plants occurs via membrane-bound Arabidopsis histidine kinases (AHKs), which transmit signals through histidinephosphor-transfer proteins and response regulators. Increasing evidence suggests that plant-associated microbes can also perceive and respond to CKs, yet knowledge of their perception pathways remains limited. This study employed a bioinformatics-based approach to investigate putative orthologous of CK signalling components in Pseudomonas fluorescens, Pseudomonas syringae, andBotrytis cinerea. Orthologous proteins with kinase, histidine phosphatase, and hybrid histidine kinase domains were identified, all containing a conserved histidine kinase (HK) domain essential for signal transduction. Notably, P.syringae AHP1 exhibited HK, HPT, and RR domains, suggesting functional versatility. Homology modelling revealed strong structural similarity of microbial orthologues to Arabidopsis CK receptors, and docking analysisdemonstrated significant CK binding, particularly by the receiver domain of theP. syringae AHK4 orthologue. Key interacting residues included leucine, glutamine, and serine. These findings provide evidence that plant-associatedmicrobes possess CK perception machinery and may respond to plant-derived CKs in the rhizosphere. Such microbial signaling capacity could have important implications for plant-microbe interactions, potentially influencing microbial colonization and plant health.
Page(s):
128-128
DOI:
DOI not available
Published:
Journal: 1st International Conference on "Recent Advances in Green Biotechnology and Climate Resilience", September 15-16, 2025, Volume: 1, Issue: 1, Year: 2025
Keywords:
arabidopsis thaliana
,
Phytohormones
,
plant microbe interactions
,
cytokinin signalling