Author(s):
1. Maria Faraza:
Gomal Center of Biochemistry & Biotechnology, Gomal University, D.I. Khan, Pakistan
2. Muhammad Badara:
Gomal Center of Biochemistry & Biotechnology, Gomal University, D.I. Khan, Pakistan
3. Seerat uz Zahraa Hafsa Khan:
Gomal Center of Biochemistry & Biotechnology, Gomal University, D.I. Khan, Pakistan
4. Asma Khan:
Gomal Center of Biochemistry & Biotechnology, Gomal University, D.I. Khan, Pakistan
Abstract:
Biofilms are the well-organized communities of microorganisms attached to biotic or abiotic surfaces and encased in the self-produced matrix of extracellular polymeric substances (EPS) mainly composed of proteins, carbohydrates and DNA. Biofilms are very difficult to eradicate by conventional antibiotics. Biofilms mostly consist of more than one bacterial species, and there occur complex interactions between species in the biofilm. One such complex interaction is between S. aureus and P. aeruginosa present in the airways of Cystic fibrosis patients and in wounds. Synergistic and antagonistic interactions occur between the two species which lead to poor clinical outcomes. It has been found that sub lethal drugs concentrations of antibiotics greatly impact the physiology of bacterial strains. Exposure of bacteria to sub lethal concentrations of antibiotics leads to disturbance in the virulence factors of bacteria including resistance, biofilm formation and eDNA production. The present study aimed to investigate the impact of 3 fluoroquinolone antibiotics including Ciprofloxacin, Levofloxacin and Ofloxacin at half of their MIC concentrations on the co-culture biofilm formation of S. aureus and P. aeruginosa at different time points. For this purpose, crystal violet binding assay was used for the biofilm quantification of 14 isolates of S. aureus which included 5 strong biofilm forming MRSA, 5 strong biofilms forming MSSA, 2 non-biofilm forming MRSA and 2 non-biofilms forming MSSA co-cultureed with P. aeruginosa. Results showed that biofilm formation was significantly reduced in 80% of strong biofilm forming MRSA and MSSA strains, while biofilm formation was observed to be increased in 75% of nonbiofilm forming isolates. The most significant results were observed after 96 hours of incubation and onwards. It is concluded from the results that sub lethal concentrations of antibiotics has major impact on the biofilm formation of co-cultured S. aureus and P. aeruginosa in a time dependent manner. Further studies in this field would help us combat the complex interactions within multi-species biofilms using sub lethal drugs concentrations.
Page(s):
59-59
DOI:
DOI not available
Published:
Journal: International Food & Nutrition Conference" August 18th-20th, 2022, University of Swat, Swat (Book of Abstracts), Volume: 1, Issue: 1, Year: 2022
Keywords:
Staphylococcus aureus
,
Biofilm formation
,
Pseudomonas aeruginosa
References:
References are not available for this document.
Citations
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