Novel Report of Fusarium oxysporum causing dry rot of carrot in Pakistan | [First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book) • 2022]

Author(s):

1. Salman Ghuffar: Vegetable Research Station Sahiwal,Pakistan

2. Muhammad Riaz: Vegetable Research Institute,AARI, Faisalabad,Pakistan

3. Sajjad Saeed: Vegetable Research Institute,AARI, Faisalabad,Pakistan

4. Waseem Abbas: Vegetable Research Institute,AARI, Faisalabad,Pakistan

5. Nasir Mehmood: The Rawalpindi Women University,Rawalpindi,Pakistan

6. Gulshan Irshad: Department of Plant Pathology PMAS, Arid Agriculture University,Rawalpindi,Pakistan

7. Umar Sabtain: Vegetable Research Station Sahiwal,Pakistan

8. Zaryab Hassan: Vegetable Research Station Sahiwal,Pakistan

9. Abdul Qayyum: Vegetable Research Station Sahiwal,Pakistan

Abstract:

-Fusarium rot or dry rot is an emerging threat to the carrot field, which is responsible for significant economic losses worldwide. The study aimed to identify Fusarium species associated with dry rot of carrot. For this purpose, a survey was conducted at Vegetable Research Station Sahiwal (VRSS) to the collection of symptomatic carrot roots. Diseased samples based on the symptoms appeared as yellowing and wilting of leaves, dry rot lesions on root surface, expressing light brown cankers with defined rounded or irregular shapes later on whitish mycelium developed on the lesions were surface sterilized with 1% sodium hypochlorite, rinsed thrice with sterile distilled water and dried by placing them on filter paper for 45 sec. Sterilized tissues (approximately 4 mm3) were excised and incubated on potato dextrose agar (PDA) medium at 25 ± 4°C. A total of ten isolates were recovered from the diseased samples. After incubation for 7 days, the morphological studies of the fungal colonies revealed the colonies as cottony with abundant aerial mycelium. In microscopic observation, Macroconidia was falciform and measured 12.1 to 30.7 (L) × 3.6 to 5.8 (W) µm with 2 to 3 septation, while elliptic Microconidia of 5.8 to 8.6 × 2.9 to 3.6 µm with none or one septate. Based on the characters, the fungus was tentatively identified as Fusarium oxysporum. For molecular characterization, DNA of the 03 isolates (ON955520, ON955053 & ON955054) were amplified with internal transcribed spacer (ITS) gene regions were sequenced. Sequence comparison revealed 99-100% genetic homology with previously reported isolates of F. oxysporum. A phylogenetic tree was constructed and analysis highlighted three diverse subtrees. After confirmation through pathogenicity test, to our knowledge, this is the first-ever detailed study of its kind to know the status of the disease along with its characterization from Pakistan. Fusarium oxysporum causing dry rot of carrot could pose a serious risk to the carrot growers of Pakistan and may require devising of appropriate management strategies. Keynote: Fusarium oxysporum, Dry rot, Carrot, Pakistan

Page(s): 0-0

DOI: DOI not available

Published: Journal: First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book), Volume: 0, Issue: 0, Year: 2022

Keywords:

Pakistan , Fusarium oxysporum , carrot , Dry rot

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