Additive Effects of Coconut Water with Various Hormones on Invitro Regeneration of Carnation (Dianthus caryophyllusl). | [Journal of Animal and Plant sciences • 2018]

Author(s):

1. M M Khatun: Plant Biotechnology Division, National Institute of Biotechnology, Savar, Dhaka, Bangladesh

2. P.K. Roy: Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Dhaka,Bangladesh

3. M. A. Razzak: Pohang University of Science and Technology, Pohang, South Korea

Abstract:

Plant needs some essential nutrient as well as growth regulator for their growth and development. In the case of Carnation (Dianthus caryophyllus L.) micro-propagation, large scale production was not possible by using the available traditional media (MS media) and method. In this study we first demonstrated the in vitro effect of coconut water (CW) together with cytokinine hormone on efficient growth of Carnation. The highest percentage of shoot induction was observed when the medium was used with coconut water (cw) at 10% +BAP 1.0 mg/l. In this case multiplication rate of carnation increased more than four times when shoot tip and nodal segment were used as an explant. So our modified media could be useful for Carnation (Dianthus caryophyllus L.) micro propagation and be a powerful media for tissue culture of Carnation. The best Carnation growth was observed in half-strength MS supplemented with 1.0 mg/l NAA for root induction. The survival rate of the transplanted plantlets was 80% in potted soil condition.

Page(s): 589-596

DOI: DOI not available

Published: Journal: Journal of Animal and Plant sciences, Volume: 28, Issue: 2, Year: 2018

Keywords:

Media , Carnation , Coconut water , Growth regulator

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