Abstract:
The main cause of deaths is the blockage of veins from blood clot that causes strokes and myocardial infarctions. Thrombolytic agents are used to remove these blood clots. This research work shows the production of fibrinolytic enzyme. Bacillus subtilis was inoculated on agar media plates and production media was used for the growth of Bacillus subtilis. Wheat bran was used as a substrate. Some of the strains of Bacillus subtilis were used for the production, isolation and characterization of fibrinolytic enzymes. The fibrinolytic enzymes were purified by the ion exchange column chromatography. The characterization of protein was done by SDS-PAGE. To find out the highest activity of these enzymes different parameters like substrate concentration, temperature and pH were applied. The enzyme produced by the isolated strain resulted in 297 U/ml with 59.51 U/mg specific activity, 4.99 mg/ml protein, 3.54 fold purification and 80% recovery. These fibrinolytic enzymes showed the highest activity at pH 7.0 and the best temperature of these enzymes was 37oC. For the production of fibrinolytic enzyme, growth of Bacillus subtilis was optimized by using different parameters such as temperature, pH, fermentation period and substrate concentration. The molecular weight of enzyme is 30 kDa.It was concluded thatwith the help of optimized conditions, highest growth of Bacillus subtilis can be achieved and low-cost fibrinolytic enzymes can be produced at industrial level in future.
Page(s):
72-72
DOI:
DOI not available
Published:
Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023